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==The structure of the catalytic domain of NcLPMO9C from the filamentous fungus Neurospora crassa==
==The structure of the catalytic domain of NcLPMO9C from the filamentous fungus Neurospora crassa==
<StructureSection load='4d7v' size='340' side='right' caption='[[4d7v]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='4d7v' size='340' side='right'caption='[[4d7v]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4d7v]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D7V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4D7V FirstGlance]. <br>
<table><tr><td colspan='2'>[[4d7v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa_OR74A Neurospora crassa OR74A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D7V FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4d7u|4d7u]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4d7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d7v OCA], [http://pdbe.org/4d7v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4d7v RCSB], [http://www.ebi.ac.uk/pdbsum/4d7v PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d7v OCA], [https://pdbe.org/4d7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d7v RCSB], [https://www.ebi.ac.uk/pdbsum/4d7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d7v ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LP9C_NEUCR LP9C_NEUCR] Lytic polysaccharide monooxygenase (LPMO) that depolymerizes crystalline and amorphous polysaccharides via the oxidation of scissile alpha- or beta-(1-4)-glycosidic bonds, yielding C4 oxidation products (PubMed:23102010, PubMed:24324265, PubMed:24733907, PubMed:26178376, PubMed:30238672, PubMed:31835532, PubMed:35080911, PubMed:36271009). Catalysis by LPMOs requires the reduction of the active-site copper from Cu(II) to Cu(I) by a reducing agent and H(2)O(2) or O(2) as a cosubstrate (PubMed:36271009). Degrades various hemicelluloses, in particular xyloglucan (PubMed:24733907, PubMed:31835532). Active on tamarind xyloglucan and konjac glucomannan (PubMed:26178376). Acts on the glucose backbone of xyloglucan, accepting various substitutions (xylose, galactose) in almost allpositions (PubMed:24733907). In contrast to all previously characterized LPMOs, which are active only on polysaccharides, is able to cleave soluble cello-oligosaccharides as short as a tetramer (PubMed:24324265). The cello-oligosaccharide products released by this enzyme contain a C4 gemdiol/keto group at the non-reducing end (PubMed:24324265). Binds to the inner wood cell wall layer and consumes enzymatically generated H(2)O(2) (PubMed:36271009).<ref>PMID:23102010</ref> <ref>PMID:24324265</ref> <ref>PMID:24733907</ref> <ref>PMID:26178376</ref> <ref>PMID:30238672</ref> <ref>PMID:31835532</ref> <ref>PMID:35080911</ref> <ref>PMID:36271009</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Borisova, A S]]
[[Category: Large Structures]]
[[Category: Dimarogona, M]]
[[Category: Neurospora crassa OR74A]]
[[Category: Eijsink, V G.H]]
[[Category: Borisova AS]]
[[Category: Isaksen, T]]
[[Category: Dimarogona M]]
[[Category: Sandgren, M]]
[[Category: Eijsink VGH]]
[[Category: Sorlie, M]]
[[Category: Isaksen T]]
[[Category: Aa9]]
[[Category: Sandgren M]]
[[Category: Catalytic domain]]
[[Category: Sorlie M]]
[[Category: Hemicellulose active]]
[[Category: Oxidoreductase]]
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