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==Crystal structure of a mycobacterial Insig homolog MvINS from Mycobacterium vanbaalenii at 1.9A resolution==
==Crystal structure of a mycobacterial Insig homolog MvINS from Mycobacterium vanbaalenii at 1.9A resolution==
<StructureSection load='4xu4' size='340' side='right' caption='[[4xu4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
<StructureSection load='4xu4' size='340' side='right'caption='[[4xu4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4xu4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_vanbaalenii_PYR-1 Mycolicibacterium vanbaalenii PYR-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XU4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.901&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4xu5|4xu5]], [[4xu6|4xu6]], [[4xu7|4xu7]], [[4xu8|4xu8]], [[4xu9|4xu9]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BNG:B-NONYLGLUCOSIDE'>BNG</scene>, <scene name='pdbligand=DDQ:DECYLAMINE-N,N-DIMETHYL-N-OXIDE'>DDQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [http://pdbe.org/4xu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [http://www.ebi.ac.uk/pdbsum/4xu4 PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xu4 OCA], [https://pdbe.org/4xu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xu4 RCSB], [https://www.ebi.ac.uk/pdbsum/4xu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xu4 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/INSIG_MYCVP INSIG_MYCVP] Diacylglycerol-binding protein.<ref>PMID:26160948</ref>  
Insulin-induced gene 1 (Insig-1) and Insig-2 are endoplasmic reticulum membrane-embedded sterol sensors that regulate the cellular accumulation of sterols. Despite their physiological importance, the structural information on Insigs remains limited. Here we report the high-resolution structures of MvINS, an Insig homolog from Mycobacterium vanbaalenii. MvINS exists as a homotrimer. Each protomer comprises six transmembrane segments (TMs), with TM3 and TM4 contributing to homotrimerization. The six TMs enclose a V-shaped cavity that can accommodate a diacylglycerol molecule. A homology-based structural model of human Insig-2, together with biochemical characterizations, suggest that the central cavity of Insig-2 accommodates 25-hydroxycholesterol, whereas TM3 and TM4 engage in Scap binding. These analyses provide an important framework for further functional and mechanistic understanding of Insig proteins and the sterol regulatory element-binding protein pathway.
 
PROTEIN STRUCTURE. Crystal structure of a mycobacterial Insig homolog provides insight into how these sensors monitor sterol levels.,Ren R, Zhou X, He Y, Ke M, Wu J, Liu X, Yan C, Wu Y, Gong X, Lei X, Yan SF, Radhakrishnan A, Yan N Science. 2015 Jul 10;349(6244):187-91. doi: 10.1126/science.aab1091. PMID:26160948<ref>PMID:26160948</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xu4" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: He, Y]]
[[Category: Large Structures]]
[[Category: Ren, R B]]
[[Category: Mycolicibacterium vanbaalenii PYR-1]]
[[Category: Wu, J P]]
[[Category: He Y]]
[[Category: Yan, C Y]]
[[Category: Ren RB]]
[[Category: Yan, N]]
[[Category: Wu JP]]
[[Category: Unknown function]]
[[Category: Yan CY]]
[[Category: Yan N]]
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