5e5c: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 5e5c is ON HOLD  until Paper Publication
==Crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1==
<StructureSection load='5e5c' size='340' side='right'caption='[[5e5c]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5e5c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E5C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e5c OCA], [https://pdbe.org/5e5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e5c RCSB], [https://www.ebi.ac.uk/pdbsum/5e5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e5c ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HYDA_PSEAE HYDA_PSEAE] Catalyzes the hydrolysis of dihydropyrimidines and of the structurally related DL-5-mono-substituted hydantoins, to produce N-carbamoyl-D-amino acids.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Dihydropyrimidinase, a tetrameric metalloenzyme, is a member of the cyclic amidohydrolase family, which also includes allantoinase, dihydroorotase, hydantoinase, and imidase. In this paper, we report the crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1 at 2.1 A resolution. The structure of P. aeruginosa dihydropyrimidinase reveals a classic (beta/alpha)8-barrel structure core embedding the catalytic dimetal center and a beta-sandwich domain, which is commonly found in the architecture of dihydropyrimidinases. In contrast to all dihydropyrimidinases, P. aeruginosa dihydropyrimidinase forms a dimer, rather than a tetramer, both in the crystalline state and in the solution. Basing on sequence analysis and structural comparison of the C-terminal region and the dimer-dimer interface between P. aeruginosa dihydropyrimidinase and Thermus sp. dihydropyrimidinase, we propose a working model to explain why this enzyme cannot be a tetramer.


Authors: Huang, C.C., Huang, Y.H., Hsieh, Y.C., Tzeng, C.T., Chen, C.J., Huang, C.Y.
Crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1: Insights into the molecular basis of formation of a dimer.,Tzeng CT, Huang YH, Huang CY Biochem Biophys Res Commun. 2016 Sep 23;478(3):1449-55. doi:, 10.1016/j.bbrc.2016.08.144. Epub 2016 Aug 26. PMID:27576201<ref>PMID:27576201</ref>


Description: Crystal structure of dihydropyrimidinase from Pseudomonas aeruginosa PAO1
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Hsieh, Y.C]]
<div class="pdbe-citations 5e5c" style="background-color:#fffaf0;"></div>
[[Category: Huang, Y.H]]
== References ==
[[Category: Huang, C.Y]]
<references/>
[[Category: Huang, C.C]]
__TOC__
[[Category: Tzeng, C.T]]
</StructureSection>
[[Category: Chen, C.J]]
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: Chen CJ]]
[[Category: Hsieh YC]]
[[Category: Huang CC]]
[[Category: Huang CY]]
[[Category: Huang YH]]
[[Category: Tzeng CT]]
Retrieved from "https://proteopedia.org/5e5c"