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'''Unreleased structure'''


The entry 3jc5 is ON HOLD
==Structure of the eukaryotic replicative CMG helicase and pumpjack motion==
<SX load='3jc5' size='340' side='right' viewer='molstar' caption='[[3jc5]], [[Resolution|resolution]] 4.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3jc5]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3JC5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3JC5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3jc5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3jc5 OCA], [https://pdbe.org/3jc5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3jc5 RCSB], [https://www.ebi.ac.uk/pdbsum/3jc5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3jc5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MCM5_YEAST MCM5_YEAST] Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity; specifically the MCM2-MCM5 association is proposed to be reversible and to mediate a open ring conformation which may facilitate DNA loading. Once loaded onto DNA, double hexamers can slide on dsDNA in the absence of ATPase activity.<ref>PMID:19896182</ref> <ref>PMID:19910535</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The CMG helicase is composed of Cdc45, Mcm2-7 and GINS. Here we report the structure of the Saccharomyces cerevisiae CMG, determined by cryo-EM at a resolution of 3.7-4.8 A. The structure reveals that GINS and Cdc45 scaffold the N tier of the helicase while enabling motion of the AAA+ C tier. CMG exists in two alternating conformations, compact and extended, thus suggesting that the helicase moves like an inchworm. The N-terminal regions of Mcm2-7, braced by Cdc45-GINS, form a rigid platform upon which the AAA+ C domains make longitudinal motions, nodding up and down like an oil-rig pumpjack attached to a stable platform. The Mcm ring is remodeled in CMG relative to the inactive Mcm2-7 double hexamer. The Mcm5 winged-helix domain is inserted into the central channel, thus blocking entry of double-stranded DNA and supporting a steric-exclusion DNA-unwinding model.


Authors: Li, H, Bai, L, Yuan, Z, Sun, J, Georgescu, RE, Liu, J, O' Donnell ME
Structure of the eukaryotic replicative CMG helicase suggests a pumpjack motion for translocation.,Yuan Z, Bai L, Sun J, Georgescu R, Liu J, O'Donnell ME, Li H Nat Struct Mol Biol. 2016 Feb 8. doi: 10.1038/nsmb.3170. PMID:26854665<ref>PMID:26854665</ref>


Description: Structure of the eukaryotic replicative CMG helicase and pumpjack motion
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Li, H, Bai, L, Yuan, Z, Sun, J, Georgescu, Re, Liu, J, O' Donnell Me]]
<div class="pdbe-citations 3jc5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bai L]]
[[Category: Georgescu RE]]
[[Category: Li H]]
[[Category: Liu J]]
[[Category: O'Donnell ME]]
[[Category: Sun J]]
[[Category: Yuan Z]]
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