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| | <StructureSection load='' size='350' side='right' caption='E. coli GroEL (green)/GroES (magenta) complex with ADP, AlF3, Mg+2 and K+ ions (PDB entry [[1pcq]])' scene='44/445432/Cv/1'> |
| [[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] | | [[Image:1pcq.png|left|200px|thumb|Crystal Structure of Chaperonin, [[1pcq]]]] |
| | '''Chaperonins''' (Cpn) are oligomeric proteins that mediate the folding of polypeptide chains. '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia]. |
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| <StructureSection load='1pcq' size='350' side='right' caption='E. coli GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions (PDB entry [[1pcq]])' scene='Chaperonin/Groel_groes_comnplex/1'> | | The most characterized Cpn are in the GroEL/GroES complex from ''Escherichia coli'' and Cpn60/Cpn10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref> The larger subunit (GroEL, Cpn60) contains 3 domains: apical, intermediate and equatorial domain. The apical domain is the one which binds the polypeptide substrate. The equatorial domains binds the nucleotide. |
| | *'''Group II Cpns''' are found during mitosis in eukaryotic cytosol and archaea. |
| | *'''Thermosome''' is a Cpn complex found in archaea.<ref>PMID:9546398</ref> |
| | *'''CCT''' or '''TRiC''' is a Cpn complex found in eukarya believed to be involved in uncontrolled proliferation.<ref>PMID:22503819</ref>, <ref>PMID:32297209</ref> |
| | *<scene name='44/445432/Cv/2'>E. coli GroEL/GroES complex</scene> (GroEL in green, GroES in magenta, PDB entry [[1pcq]]). |
| | *<scene name='44/445432/Cv/3'>GroEL/GroES complex with ADP, AlF3, Mg+2 and K+ ions</scene>. |
| | *<scene name='44/445432/Cv/5'>K+ ion coordination site</scene>. GroEL in cyan. |
| | *<scene name='44/445432/Cv/6'>AlF3 binding site</scene>. |
| | *<scene name='44/445432/Cv/7'>Mg+2 ion coordination site</scene>. |
| | *<scene name='44/445432/Cv/9'>ADP binding site</scene>. |
| | *<scene name='44/445432/Cv/10'>Whole binding site</scene>. |
| | *See also [[Chaperones]].<br /> |
| | *For GroEl in Hebrew see [[Sand box groel]]. |
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| '''Chaperonins''' (CPN) are oligomeric proteins that mediate the folding of polypeptide chains. '''Group I CPN''' are found in bacteria, chloroplasts and mitochondria. For an introductory overview, see [http://en.wikipedia.org/wiki/Chaperonins Chaperonins in Wikipedia].
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| The most characterized CPN are in the GroEL/GroES complex from ''Escherichia coli'' and CPN60/CPN10 from ''Thermus thermophilus''.<ref>PMID:18987317</ref> The larger subunit (GroEL, CPN60) contains 3 domains. The apical domain is the one which binds the substrate. '''Group II CPNs''' are found in eukaryotic cytosol and archaea. '''Thermosome''' is a CPN complex found in archaea.<ref>PMID:9546398</ref> '''CCT''' is a CPN complex found in eukarya.<ref>PMID:22503819</ref>
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| See also [[Chaperones]].
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| </StructureSection>
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| == 3D Structures of Chaperonin == | | == 3D Structures of Chaperonin == |
| | [[Chaperonin 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | == Files for 3D printer == |
| {{#tree:id=OrganizedByTopic|openlevels=0|
| | <i class="fas fa-cubes"></i> Asymmetric Chaperonin Complex GroEL/GroES by [[User:Marius Mihasan|Marius Mihasan]] [https://3dprint.nih.gov/discover/3dpx-017057 <i class="fas fa-download"></i>] |
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| *'''Group I'''
| | </StructureSection> |
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| *''Large subunit''
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| **[[3m6c]] – MtGroEL1 apical domain – ''Mycobacterium tuberculosis''<BR />
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| **[[1sjp]], [[3rtk]] – MtGroEL2 residues 42-539<BR />
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| **[[3fbh]], [[2eu1]], [[1j4z]], [[1kpo]], [[1oel]], [[1grl]], [[2yey]] – EcGroEL (mutant) - ''Escherichia coli''<BR />
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| **[[3e76]], [[2nwc]], [[1xck]], [[1ss8]], [[4hel]] – EcGroEL<BR />
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| **[[3c9v]], [[3cau]], [[2ynj]] – EcGroEL – Cryo EM<BR />
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| **[[2c7e]], [[1gr5]], [[4aaq]] - EcGroEL (mutant) – Cryo EM<BR />
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| **[[1dk7]], [[1la1]] - EcGroEL apical domain<br />
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| **[[1fy9]], [[1fya]], [[1kid]], [[1jon]], [[3vz6]], [[3vz7]], [[3vz8]] – EcGroEL apical domain (mutant) <BR />
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| **[[1srv]] – TtCPN60 apical domain - ''Thermus thermophilus''<BR />
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| **[[3osx]] - CPN60 apical domain – ''Xenorhabdus nematophila''<br />
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| **[[1iok]] – CPN60 – ''Paracoccus denitrificans''
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| *''Large subunit binary complex''
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| **[[1kp8]] - EcGroEL (mutant) + ATP<BR />
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| **[[4aar]], [[4aas]], [[4aau]], [[4ab2]], [[4ab3]] - EcGroEL (mutant) + ATP – Cryo EM<br />
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| **[[1sx3]] - EcGroEL + ATP<BR />
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| **[[1sx4]] - EcGroEL + ADP<BR />
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| **[[4ki8]] - EcGroEL (mutant) + ADP<br />
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| **[[1mnf]] – EcGroEL + polypeptide<BR />
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| **[[2cgt]] – EcGroEL + capsid assbly protein GP31 – EM<BR />
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| **[[1dkd]] - EcGroEL apical domain + polypeptide
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| *''Small subunit''
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| **[[3nx6]] – GroES residues 40-134 (mutant) – ''Xanthomonas oryzae''<BR />
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| **[[1wnr]] – TtCPN10 residues 1-94<BR />
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| **[[1p3h]], [[1hx5]] – MtCPN10<BR />
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| **[[1p82]], [[1p83]] - MtCPN10 residues 1-25 - NMR<BR />
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| **[[1egs]] - GroES residues 19-27 - NMR
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| *''Large + small subunit''
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| **[[1svt]], [[1pcq]] - EcGroEL + GroES<BR />
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| **[[1pf9]], [[1aon]], [[4pko]] - EcGroEL + GroES + ADP<BR />
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| **[[3wvl]] - EcGroEL (mutant) + GroES + ATP<br />
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| **[[2c7c]], [[2c7d]] - EcGroEL + GroES – Cryo EM<BR />
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| **[[1gru]] - EcGroEL + GroES + ATP + ADP – Cryo EM<BR />
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| **[[3zpz]], [[3zq0]], [[3zq1]] - EcGroEL + GroES + ADP – Cryo EM<br />
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| **[[1we3]] – TtCPN60 + CPN10<BR />
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| **[[1wf4]], [[4v4o]], [[4pkn]] - TtCPN60 + CPN10 + ADP
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| *'''Group II'''
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| **[[3izh]], [[3izi]], [[3izj]], [[3izk]], [[3izl]], [[3izm]], [[3izn]], [[3los]], [[3iyf]], [[3j3x]] – MmCPN – ''Methanococcus maripaludis'' – EM<BR />
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| **[[3kfb]], [[3kfe]], [[3kfk]] – MmCPN<BR />
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| **[[3j02]], [[3j03]] – MmCPN (mutant) – Cryo EM<br />
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| **[[3ruq]] - MmCPN + ADP<br />
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| **[[3rus]] - MmCPN (mutant) + ADP<br />
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| **[[3ruw]] - MmCPN (mutant) + ADP-AlF3<br />
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| **[[3ruv]] - MmCPN (mutant) + ATP analog<br />
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| *'''CCT'''
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| **[[2xsm]], [[4b2t]] – bCPN CCT – bovine<BR />
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| **[[3p9d]], [[3p9e]], [[4d8q]], [[4d8r]], [[4aol]], [[4apk]] – CPN CCT - yeast<br />
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| **[[3iyg]], [[4a0o]] - bCPN CCT – Cryo EM<BR />
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| **[[4a0v]] - bCPN CCT + AMPPNP – Cryo EM<br />
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| **[[4a0w]] - bCPN CCT + ADP + AlF3 – Cryo EM<br />
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| **[[4a13]] - bCPN CCT + ADP – Cryo EM<br />
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| **[[3ktt]] - bCPN CCT β subunit – Cryo EM<BR />
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| **[[1gml]], [[1gn1]] - CPN CCT γ subunit apical domain – mouse
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| *'''Hsp33'''
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| See [[Heat Shock Proteins]]
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| *'''Thermosome'''
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| **[[1a6d]] - TaTherm α+β subunits – ''Thermoplasma acidophilum''<BR />
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| **[[1a6e]] - TaTherm α+β subunits + ADP<BR />
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| **[[1ass]], [[1asx]] - TaTherm α apical domain<BR />
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| **[[1e0r]] – TaTherm β apical domain<br />
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| **[[3ko1]] – AtTherm α subunit– ''Acidianus tengchongensis''<br />
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| **[[3j1b]], [[3j1c]], [[3j1e]] - AtTherm α subunit – Cryo EM <br />
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| **[[3j1f]] - AtTherm β subunit + ATP – Cryo EM <br />
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| **[[3aq1]] – Therm – ''Methanococcoides burtonii''<br />
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| **[[1q2v]], [[1q3r]] – TkTherm α subunit (mutant) – ''Thermococcus KS-1''<br />
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| **[[1q3q]] - TkTherm α subunit (mutant) + AMP-PNP<br />
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| **[[1q3s]] - TkTherm α subunit (mutant) + ADP<br />
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| **[[1lep]] – CPN-10 – ''Mycobacterium leprae''
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category:Topic Page]] | | [[Category:Topic Page]] |
| | [[Category:3D printer files]] |
