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| <StructureSection load='1oab' size='340' side='right' caption='Yeast DAHP synthase complex with Mn+2 ion and PEP (PDB code [[1oab]])' scene=''> | | <StructureSection load='' size='350' side='right' caption='E. coli DAHP synthase complex with PEP, phenylalanine, sulfate and Mn+2 ion (PDB code [[1kfl]])' scene='70/708806/Cv/1'> |
| == Function == | | == Function == |
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| '''DAHP synthase''' or '''3-deoxy-D-arabino-heptulosonate 7-phosphate synthase''' (DAHPS) catalyzes the conversion of phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) and phosphate. DAHPS is part of the shikimate pathway. DAHPS requires a bivalent metal ion cofactor for normal activity. DAHPS is a tetramer. DAHPS exhibits feedback inhibition by aromatic amino acids like tyrosine, phenylalanine and tryptophan.<ref>PMID:1682314</ref> | | '''DAHP synthase''' or '''3-deoxy-D-arabino-heptulosonate 7-phosphate synthase''' or '''phospho-2-dehydro-3-deoxyheptonate aldolase''' (DAHPS) catalyzes the conversion of phosphoenolpyruvate (PEP) and D-erythrose 4-phosphate to 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) and phosphate. DAHPS is part of the shikimate pathway. DAHPS requires a bivalent metal ion cofactor for normal activity. <scene name='70/708806/Cv/6'>DAHPS is a tetramer</scene>. DAHPS exhibits feedback inhibition by aromatic amino acids like tyrosine, phenylalanine and tryptophan.<ref>PMID:1682314</ref> |
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| == Structural highlights == | | == Structural highlights == |
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| The bivalent metal is bound to a Cys-X-X-His motif. The DAHPS active site is located in a channel at the C-terminal of the enzyme where the substrate (PEP), inhibitor (phenylalanine) and the metal ion (Mn+2) are seen.<ref>PMID:12126632</ref>
| | The DAHPS active site is located in a channel at the C-terminal of the enzyme where the <scene name='70/708806/Cv/7'>substrate (PEP)</scene>, <scene name='70/708806/Cv/8'>inhibitor (phenylalanine)</scene> and <scene name='70/708806/Cv/9'>metal ion (Mn+2)</scene> are seen. The bivalent metal is bound to a Cys-X-X-His motif.<ref>PMID:12126632</ref> |
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| </StructureSection>
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| == 3D Structures of DAHP synthase == | | == 3D Structures of DAHP synthase == |
| | [[DAHP synthase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *DAHP synthase
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| **[[1vr6]] - TmDAHPS – ''Thermotoga maritima''<br />
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| **[[3pg8]] - TmDAHPS catalytic domain<br />
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| **[[1ofp]] - yDAHPS – yeast<br />
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| **[[1ofb]], [[1ofq]] - yDAHPS + Mn<br />
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| **[[4uma]], [[4umb]], [[4umc]] - NmDAHPS + Mn – ''Nisseria meningitidis''<br />
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| **[[4ixx]] - NmDAHPS (mutant) + Mn <br />
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| **[[3tqk]] - DAHPS + Mn – ''Francisella tularensis''<br />
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| *DAHP synthase binary complex
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| **[[4hsn]] - NmDAHPS + Mn + PEP <br />
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| **[[4c1k]] - PfDAHPS + Cd + PEP – ''Pyrococcus furiosus''<br />
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| **[[1zco]] - PfDAHPS + Mn + PEP <br />
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| **[[1of6]] - yDAHPS + Mn + tyrosine <br />
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| **[[1hfb]] - yDAHPS + PEP <br />
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| **[[1oab]] - yDAHPS + Mn + PEP <br />
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| **[[1ofa]] - yDAHPS + Co + PEP <br />
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| **[[1ofr]] - yDAHPS + Mn + phenylalanine<br />
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| **[[1og0]] - yDAHPS (mutant) + Mn + phenylalanine <br />
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| **[[2b7o]] - MtDAHPS + Mn + PEP – ''Mycobacterium tuberculosis''<br />
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| **[[3nud]] - MtDAHPS + phenylalanine<br />
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| **[[2ypo]] - MtDAHPS + Mn + phenylalanine<br />
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| **[[3nue]] - MtDAHPS + Mn + tryptophan<br />
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| **[[2ypp]] - MtDAHPS + Mn + tyrosine<br />
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| **[[3nv8]] - MtDAHPS + Mn + PEP <br />
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| **[[3pfp]] - MtDAHPS + Mn + inhibitor <br />
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| **[[1qr7]] - EcDAHPS + Pb + PEP – ''Escherichia coli''<br />
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| **[[1gg1]] - EcDAHPS + Mn + phosphoglycolate <br />
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| **[[1n8f]] – EcDAHPS (mutant) + Mn + PEP<br />
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| **[[1ofo]] - yDAHPS + phosphoglycolate
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| **[[1vs1]] - DAHPS + Mn + PEP – ''Aeropyrum pernix''<br />
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| **[[2w19]] - MtDAHPS + chorismate mutase <br />
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| *DAHP synthase ternary complex
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| **[[1of8]] - yDAHPS + Co + PEP + glycerol-3-phosphate<br />
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| **[[1rzm]] - TmDAHPS + Cd + PEP + erythrose-4-phosphate<br />
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| **[[3pg9]] – TmDAHPS + tyrosine + inhibitor<br />
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| **[[1kfl]] - EcDAHPS + Mn + PEP + phenylalanine <br />
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| **[[4hso]] - MnDAHPS (mutant) + Mn + PEP + tyrosine <br />
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| **[[2w1a]] - MtDAHPS + Mn + TSA + chorismate mutase <br />
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| **[[3kgf]], [[3rzi]] - MtDAHPS + Mn + phenylalanine + tryptophan<br />
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| **[[2ypq]] - MtDAHPS + Mn + tyrosine + tryptophan<br />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| | [[Category:Topic Page]] |
