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| <StructureSection load='4oyl' size='350' side='right' caption='Structure of cutinase complex with inhibitor E600 (PDB entry [[4oyl]])' scene='55/551226/Cv/1'> | | <StructureSection load='4oyl' size='350' side='right' caption='Structure of cutinase (PDB entry [[4oyl]])' scene='55/551226/Cv/1'> |
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| '''Cutinase''' (CUT) catalyzes the conversion of cutin to cutin monomers. Cutin is a main component of the plant cuticle. Polymeric cutin which is composed of hydroxyl and hydroxyepoxy fatty acids is found in the outer layer of plants. CUT is found in fungi and bacteria. CUT degrades the cutin enabling pathogens’ penetration into plant cells. CUT is inhibited by organophosphates like E600 (diethyl-p-nitrophenyl phosphate) and organophosphonates.<ref>PMID:9175860</ref> | | '''Cutinase''' (CUT) or '''α/β hydrolase family protein''' the conversion of cutin to cutin monomers. Cutin is a main component of the plant cuticle. Polymeric cutin which is composed of hydroxyl and hydroxyepoxy fatty acids is found in the outer layer of plants. CUT is found in fungi and bacteria. CUT degrades the cutin enabling pathogens’ penetration into plant cells. CUT is inhibited by organophosphates like E600 (diethyl-p-nitrophenyl phosphate) and organophosphonates.<ref>PMID:9175860</ref> |
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| == Relevance == | | == Relevance == |
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| Inhibition of CUT can prevent fungal plant infection. | | Inhibition of CUT can prevent fungal plant infection<ref>PMID:17758793</ref>. |
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| == Structural highlights == | | == Structural highlights == |
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| Upon CUT inhibition by organophosphates the <scene name='55/551226/Cv/2'>catalytic triad of Ser-His-Asp exhibits esterification of Ser and ethylation of His</scene>.<ref>PMID:24832484</ref> | | Upon CUT inhibition by organophosphates the <scene name='55/551226/Cv/3'>catalytic triad of Ser-His-Asp exhibits esterification of Ser and ethylation of His</scene>.<ref>PMID:24832484</ref> |
| </StructureSection>
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| == 3D Structures of cutinase == | | == 3D Structures of cutinase == |
| | [[Cutinase 3D structures]] |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | </StructureSection> |
| {{#tree:id=OrganizedByTopic|openlevels=0|
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| *Cutinase
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| **[[1cus]], [[2cut]], [[1oxm]], [[1cex]], [[1agy]], [[3qpa]] – NhCUT – ''Nectria haematococca''<BR />
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| **[[1ffa]], [[1ffb]], [[1ffc]], [[1ffd]], [[1ffe]], [[1cua]], [[1cub]], [[1cuc]], [[1cud]], [[1cue]], [[1cuf]], [[1cug]], [[1cuh]], [[1cui]], [[1cuj]], [[1cuu]], [[1cuv]], [[1cuw]], [[1cux]], [[1cuy]], [[1cuz]], [[1xza]], [[1xzb]], [[1xzc]], [[1xzd]], [[1xze]], [[1xzf]], [[1xzg]], [[1xzh]], [[1xzi]], [[1xzj]] – NhCUT (mutant)<BR />
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| **[[3dcn]] – GcCUT –'' Glomerella cingulata''<BR />
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| **[[3gbs]] – CUT – ''Aspergillus oryzae'' <BR />
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| **[[3esc]] – CUT – ''Fusarium solani''<br />
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| **[[4cg1]], [[4cg2]], [[4cg3]] – CUT – ''Thermobifida fusca''<br />
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| **[[4oyy]] – HiCUT – ''Humicola insolens''<BR />
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| **[[4wfi]], [[4wfj]], [[4wfk]] – CUT (mutant) – ''Sacchromonospora viridis''<BR />
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| *Cutinase complex with inhibitor
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| **[[1xzl]] – NhCUT + hexylphosphonate ethyl ester<BR />
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| **[[1xzk]] – NhCUT + di-isopropyl phosphate <BR />
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| **[[1xzm]] – NhCUT + undecyl chloro phosphonate ester<BR />
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| **[[3dd5]] – GcCUT + E600 <BR />
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| **[[3dea]] – GcCUT + PETFP <BR />
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| **[[3ef3]], [[3esa]], [[3esb]], [[3eac]], [[3esd]] – NhCUT + Pt inhibitor <BR />
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| **[[3qpc]] – NhCUT + paraoxon <BR />
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| **[[3qpd]] – NhCUT + phosphoserine <BR />
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| **[[4oyl]] – HiCUT + ethyl phosphate <BR />
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| }}
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| == References == | | == References == |
| <references/> | | <references/> |
| [[Category: Topic Page]] | | [[Category: Topic Page]] |
