5fd3: Difference between revisions

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New page: '''Unreleased structure''' The entry 5fd3 is ON HOLD Authors: Marceau, A.H., Felthousen, J.G., Goetsch, P.D., Lee, H., Tripathi, S.M., Strome, S. , Litovchick, L., Rubin, S.M. Descript...
 
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'''Unreleased structure'''


The entry 5fd3 is ON HOLD
==Structure of Lin54 tesmin domain bound to DNA==
<StructureSection load='5fd3' size='340' side='right'caption='[[5fd3]], [[Resolution|resolution]] 2.42&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5fd3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FD3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FD3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.42&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fd3 OCA], [https://pdbe.org/5fd3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fd3 RCSB], [https://www.ebi.ac.uk/pdbsum/5fd3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fd3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LIN54_HUMAN LIN54_HUMAN] Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The MuvB complex recruits transcription factors to activate or repress genes with cell cycle-dependent expression patterns. MuvB contains the DNA-binding protein LIN54, which directs the complex to promoter cell cycle genes homology region (CHR) elements. Here we characterize the DNA-binding properties of LIN54 and describe the structural basis for recognition of a CHR sequence. We biochemically define the CHR consensus as TTYRAA and determine that two tandem cysteine rich regions are required for high-affinity DNA association. A crystal structure of the LIN54 DNA-binding domain in complex with a CHR sequence reveals that sequence specificity is conferred by two tyrosine residues, which insert into the minor groove of the DNA duplex. We demonstrate that this unique tyrosine-mediated DNA binding is necessary for MuvB recruitment to target promoters. Our results suggest a model in which MuvB binds near transcription start sites and plays a role in positioning downstream nucleosomes.


Authors: Marceau, A.H., Felthousen, J.G., Goetsch, P.D., Lee, H., Tripathi, S.M., Strome, S. , Litovchick, L., Rubin, S.M.
Structural basis for LIN54 recognition of CHR elements in cell cycle-regulated promoters.,Marceau AH, Felthousen JG, Goetsch PD, Iness AN, Lee HW, Tripathi SM, Strome S, Litovchick L, Rubin SM Nat Commun. 2016 Jul 28;7:12301. doi: 10.1038/ncomms12301. PMID:27465258<ref>PMID:27465258</ref>


Description: Structure of Lin54 tesmin domain bound to DNA
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Marceau, A.H]]
<div class="pdbe-citations 5fd3" style="background-color:#fffaf0;"></div>
[[Category: Rubin, S.M]]
== References ==
[[Category: Tripathi, S.M]]
<references/>
[[Category: Strome, S. , Litovchick, L]]
__TOC__
[[Category: Felthousen, J.G]]
</StructureSection>
[[Category: Lee, H]]
[[Category: Homo sapiens]]
[[Category: Goetsch, P.D]]
[[Category: Large Structures]]
[[Category: Felthousen JG]]
[[Category: Goetsch PD]]
[[Category: Lee H]]
[[Category: Litovchick L]]
[[Category: Marceau AH]]
[[Category: Rubin SM]]
[[Category: Strome S]]
[[Category: Tripathi SM]]
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