5fqu: Difference between revisions
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The | ==Orthorhombic crystal structure of of PlpD (selenomethionine derivative)== | ||
<StructureSection load='5fqu' size='340' side='right'caption='[[5fqu]], [[Resolution|resolution]] 2.74Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5fqu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FQU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FQU FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.74Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fqu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fqu OCA], [https://pdbe.org/5fqu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fqu RCSB], [https://www.ebi.ac.uk/pdbsum/5fqu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fqu ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9HYQ6_PSEAE Q9HYQ6_PSEAE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The type V secretion system is a macromolecular machine employed by a number of bacteria to secrete virulence factors into the environment. The human pathogen Pseudomonas aeruginosa employs the newly described type Vd secretion system to secrete a soluble variant of PlpD, a lipase of the patatin-like family synthesized as a single macromolecule that also carries a polypeptide transport-associated domain and a 16-stranded beta-barrel. Here we report the crystal structure of the secreted form of PlpD in its biologically active state. PlpD displays a classical lipase alpha/beta hydrolase fold with a catalytic site located within a highly hydrophobic channel that entraps a lipidic molecule. The active site is covered by a flexible lid, as in other lipases, indicating that this region in PlpD must modify its conformation in order for catalysis at the water-lipid interface to occur. PlpD displays phospholipase A1 activity and is able to recognize a number of phosphatidylinositols and other phosphatidyl analogs. PlpD is the first example of an active phospholipase secreted through the type V secretion system, for which there are more than 200 homologs, revealing details of the lipid destruction arsenal expressed by P. aeruginosa in order to establish infection. | |||
Structural Basis of Lipid Targeting and Destruction by the Type V Secretion System of Pseudomonas aeruginosa.,da Mata Madeira PV, Zouhir S, Basso P, Neves D, Laubier A, Salacha R, Bleves S, Faudry E, Contreras-Martel C, Dessen A J Mol Biol. 2016 May 8;428(9 Pt A):1790-803. doi: 10.1016/j.jmb.2016.03.012. Epub, 2016 Mar 21. PMID:27012424<ref>PMID:27012424</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
[[Category: | <div class="pdbe-citations 5fqu" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Bleves | __TOC__ | ||
[[Category: Contreras-Martel | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Pseudomonas aeruginosa PAO1]] | ||
[[Category: Laubier | [[Category: Basso P]] | ||
[[Category: | [[Category: Bleves S]] | ||
[[Category: | [[Category: Contreras-Martel C]] | ||
[[Category: Dessen A]] | |||
[[Category: Faudry E]] | |||
[[Category: Laubier A]] | |||
[[Category: Neves D]] | |||
[[Category: Salacha R]] | |||
[[Category: Vinicius da Mata Madeira P]] | |||
[[Category: Zouhir S]] | |||
Latest revision as of 13:19, 26 July 2023
Orthorhombic crystal structure of of PlpD (selenomethionine derivative)
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