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==Phototoxic Fluorescent Protein KillerOrange==
==Phototoxic Fluorescent Protein KillerOrange==
<StructureSection load='4zfs' size='340' side='right' caption='[[4zfs]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
<StructureSection load='4zfs' size='340' side='right'caption='[[4zfs]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4zfs]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ZFS FirstGlance]. <br>
<table><tr><td colspan='2'>[[4zfs]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Hydrozoa Hydrozoa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZFS FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4M9:(4Z)-4-IMINO-4-[(4Z)-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-1-(2-OXOETHYL)-4,5-DIHYDRO-1H-IMIDAZOL-2-YL]BUTANAMIDE'>4M9</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4zbl|4zbl]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4M9:(4Z)-4-IMINO-4-[(4Z)-4-(1H-INDOL-3-YLMETHYLIDENE)-5-OXO-1-(2-OXOETHYL)-4,5-DIHYDRO-1H-IMIDAZOL-2-YL]BUTANAMIDE'>4M9</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zfs OCA], [http://pdbe.org/4zfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zfs RCSB], [http://www.ebi.ac.uk/pdbsum/4zfs PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zfs OCA], [https://pdbe.org/4zfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zfs RCSB], [https://www.ebi.ac.uk/pdbsum/4zfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zfs ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q2TCH5_9CNID Q2TCH5_9CNID]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. Here, we present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKillerOrange, at 1.81 and 1.57 A resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the beta-barrel exterior and enabling transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers.
Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.,Pletneva NV, Pletnev VZ, Sarkisyan KS, Gorbachev DA, Egorov ES, Mishin AS, Lukyanov KA, Dauter Z, Pletnev S PLoS One. 2015 Dec 23;10(12):e0145740. doi: 10.1371/journal.pone.0145740., eCollection 2015. PMID:26699366<ref>PMID:26699366</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4zfs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pletnev, S]]
[[Category: Hydrozoa]]
[[Category: Pletnev, V Z]]
[[Category: Large Structures]]
[[Category: Pletneva, N V]]
[[Category: Pletnev S]]
[[Category: Chromophore-assisted light inactivation]]
[[Category: Pletnev VZ]]
[[Category: Fluorescent protein]]
[[Category: Pletneva NV]]
[[Category: Genetically encoded photosensitizer]]
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