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==Crystal Structure of Highly Active Mutant of Bacillus sp. TB-90 Urate Oxidase==
==Crystal Structure of Highly Active Mutant of Bacillus sp. TB-90 Urate Oxidase==
<StructureSection load='4xfp' size='340' side='right' caption='[[4xfp]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
<StructureSection load='4xfp' size='340' side='right'caption='[[4xfp]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4xfp]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XFP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4XFP FirstGlance]. <br>
<table><tr><td colspan='2'>[[4xfp]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._TB-90 Bacillus sp. TB-90]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XFP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XFP FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AZA:8-AZAXANTHINE'>AZA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wlv|3wlv]], [[1j2g|1j2g]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZA:8-AZAXANTHINE'>AZA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Factor_independent_urate_hydroxylase Factor independent urate hydroxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xfp OCA], [https://pdbe.org/4xfp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xfp RCSB], [https://www.ebi.ac.uk/pdbsum/4xfp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xfp ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4xfp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xfp OCA], [http://pdbe.org/4xfp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4xfp RCSB], [http://www.ebi.ac.uk/pdbsum/4xfp PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PUCL_BACSB PUCL_BACSB]] Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin (By similarity).  
[https://www.uniprot.org/uniprot/PUCL_BACSB PUCL_BACSB] Catalyzes two steps in the degradation of uric acid, i.e. the oxidation of uric acid to 5-hydroxyisourate (HIU) and the stereoselective decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The optimal activity of Bacillus sp. TB-90 urate oxidase (BTUO) is 45 degrees C, but this enzyme is one of the most thermostable urate oxidases. A marked increase (&gt;10 degrees C) in its thermal stability is induced by high concentrations (0.8-1.2 M) of sodium sulfate. Calorimetric measurements and size exclusion chromatographic analyses suggested that sulfate-induced thermal stabilization is related to the binding of a sulfate anion that repressed the dissociation of BTUO tetramers into dimers. To determine the sulfate binding site, the crystal structure was determined at 1.75 A resolution. The bound sulfate anion was found at the subunit interface of the symmetrical related subunits and formed a salt bridge with two Arg298 residues in the flexible loop that is involved in subunit assembly. Site-directed mutagenesis of Arg298 to Glu was used to extensively characterize the sulfate binding site at the subunit interface. The network of charged hydrogen bonds via the bound sulfate is suggested to contribute significantly to the thermal stabilization of both subunit dimers and the tetrameric assembly of BTUO. Knowledge of the mechanism of salt-induced stabilization will help to develop new strategies for enhancing protein thermal stabilization.
 
Intersubunit Salt Bridges with a Sulfate Anion Control Subunit Dissociation and Thermal Stabilization of Bacillus sp. TB-90 Urate Oxidase.,Hibi T, Hayashi Y, Fukada H, Itoh T, Nago T, Nishiya Y Biochemistry. 2014 Jun 13. PMID:24897238<ref>PMID:24897238</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==See Also==
</div>
*[[Urate oxidase 3D structures|Urate oxidase 3D structures]]
<div class="pdbe-citations 4xfp" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Factor independent urate hydroxylase]]
[[Category: Bacillus sp. TB-90]]
[[Category: Hayashi, Y]]
[[Category: Large Structures]]
[[Category: Hibi, T]]
[[Category: Hayashi Y]]
[[Category: Itoh, T]]
[[Category: Hibi T]]
[[Category: Kawamura, A]]
[[Category: Itoh T]]
[[Category: Enzyme]]
[[Category: Kawamura A]]
[[Category: Flexible loop]]
[[Category: Oxidoreductase]]
[[Category: Protein engineering]]

Latest revision as of 15:27, 8 November 2023

Crystal Structure of Highly Active Mutant of Bacillus sp. TB-90 Urate Oxidase

4xfp, resolution 1.66Å

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