5f99: Difference between revisions
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The | ==X-ray Structure of the MMTV-A Nucleosome Core Particle== | ||
<StructureSection load='5f99' size='340' side='right'caption='[[5f99]], [[Resolution|resolution]] 2.63Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5f99]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Mouse_mammary_tumor_virus Mouse mammary tumor virus] and [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F99 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.63Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f99 OCA], [https://pdbe.org/5f99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f99 RCSB], [https://www.ebi.ac.uk/pdbsum/5f99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f99 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/H32_XENLA H32_XENLA] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for two similar alpha-satellite sequences and a sequence (601) selected in vitro have been visualized bound in the nucleosome core. Here we report the 2.6-A resolution X-ray structure of a nucleosome core particle containing the DNA sequence of nucleosome A of the 3'-LTR of the mouse mammary tumor virus (147 bp MMTV-A). To our knowledge, this is the first nucleosome core particle structure containing a promoter sequence and crystallized from Mg2+ ions. It reveals sequence-dependent DNA conformations not seen previously, including kinking into the DNA major groove. | |||
X-ray structure of the MMTV-A nucleosome core.,Frouws TD, Duda SC, Richmond TJ Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201524607. PMID:26787910<ref>PMID:26787910</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5f99" style="background-color:#fffaf0;"></div> | ||
[[Category: Frouws | |||
==See Also== | |||
*[[Histone 3D structures|Histone 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Mouse mammary tumor virus]] | |||
[[Category: Xenopus laevis]] | |||
[[Category: Frouws TD]] | |||
[[Category: Richmond TJ]] | |||
Latest revision as of 06:42, 19 July 2023
X-ray Structure of the MMTV-A Nucleosome Core Particle
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