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==Plant Viral Protein p19 Suppression of RNA Silencing==
==Plant Viral Protein p19 Suppression of RNA Silencing==
<StructureSection load='' size='400' side='right' caption='p19 dimer and siRNA [[1rpu]]' scene='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>
<StructureSection load='' size='350' side='right' caption='p19 dimer and siRNA [[1rpu]]' scene='User:Wayne_Decatur/Plant_Viral_Protein_p19_Suppression_of_RNA_Silencing/View1/2'>
==Background==
==Background==
[http://en.wikipedia.org/wiki/RNA_Silencing RNA silencing] is a gene inactivation system in many eukaryotes that relies on tiny RNAs as the targeting molecules. One function of RNA silencing, which is also called [http://en.wikipedia.org/wiki/Post-transcriptional_gene_silencing post-transcriptional gene silencing (PTGS)] or [[RNA Interference|RNA interference]] (RNAi), is to act in surveillance against molecular parasites, such as viruses. Double-stranded RNA triggers the RNA silencing pathway and most plant viruses use a double-stranded RNA to replicate their genome. Various plant viruses have developed evasion techniques to circumvent this surveillance system. In one such evasion strategy, the plant viral protein p19 suppresses a plant's anti-viral RNA silencing response. p19 binds with high affinity to the double-stranded RNA silencing mediator, called [http://en.wikipedia.org/wiki/Small_interfering_RNA siRNA], and this binding sequesters the siRNA, preventing its participation in later steps of RNA silencing.   
[http://en.wikipedia.org/wiki/RNA_Silencing RNA silencing] is a gene inactivation system in many eukaryotes that relies on tiny RNAs as the targeting molecules. One function of RNA silencing, which is also called [http://en.wikipedia.org/wiki/Post-transcriptional_gene_silencing post-transcriptional gene silencing (PTGS)] or [[RNA Interference|RNA interference]] (RNAi), is to act in surveillance against molecular parasites, such as viruses. Double-stranded RNA triggers the RNA silencing pathway and most plant viruses use a double-stranded RNA to replicate their genome. Various plant viruses have developed evasion techniques to circumvent this surveillance system. In one such evasion strategy, the plant viral protein p19 suppresses a plant's anti-viral RNA silencing response. p19 binds with high affinity to the double-stranded RNA silencing mediator, called [http://en.wikipedia.org/wiki/Small_interfering_RNA siRNA], and this binding sequesters the siRNA, preventing its participation in later steps of RNA silencing.   
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}


[[1rpu]] – P19 + SiRNA – Carnation italian ringspot virus<br />
[[1rpu]], [[6bjv]] – CirvP19 + SiRNA – Carnation italian ringspot virus<br />
[[1rpf]], [[4jk0]], [[4kq0]] - TbsvP19 (mutant) + SiRNA –Tomato bushy stunt virus<br />
[[6bjh]], [[6bjg]] - CirvP19 (mutant) + SiRNA<br />
[[3lzl]],[[3lzn]],[[3lzo]],[[3lzp]],[[3lzq]],[[3lzr]] – P19 – ''Campylobacter jejuni''<br />
[[4j39]], [[4j5v]],  [[4jgn]], [[4jnx]], [[4knq]], [[4ktg]] - TbsvP19 + SiRNA - Tomato bushy stunt virus <br />
[[4j39]], [[4j5v]], [[4jgn]], [[4jnx]], [[4knq]], [[4ktg]] - TbsvP19 + SiRNA<br />
[[1rpf]], [[4jk0]], [[4kq0]] - TbsvP19 (mutant) + SiRNA<br />
[[3lzl]],[[3lzn]],[[3lzo]],[[3lzp]],[[3lzq]],[[3lzr]], [[5i0w]], [[5i0v]]  – P19 – ''Campylobacter jejuni''<br />
[[5dof]] - TtP19 – ''Tetrahymena thermophila''<br />
[[5doi]] - TtP19 + P45 <br />
[[6cus]] - RsvP19 M-domain (mutant) + inositol-hexakisphosphate – Rous sarcoma virus - NMR<br />
[[6cv8]], [[6cw4]] - RsvP19 M-domain (mutant) + inositol-trisphosphate – Rous sarcoma virus - NMR<br />
[[8h2i]] - RbcvP19 in capsid – Paramecium bursara chlorella virus <br />


==Related Structures and Topics==
==Related Structures and Topics==
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==Additional Literature and Resources==
==Additional Literature and Resources==
*[http://www-unix.oit.umass.edu/~wdecatur/p19tutorial/ Tour of p19 bound to an siRNA] by Wayne Decatur, in an exploration-friendly interface that is adapted from Eric Martz's [http://molvis.sdsc.edu/fgij/index.htm FirstGlance in Jmol]<br>
*Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b., Chen HY, Yang J, Lin C, Yuan YA, EMBO Rep. 2008 Aug;9(8):754-60. Epub 2008 Jul 4.PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18600235 18600235]<br>
*Structural basis for RNA-silencing suppression by Tomato aspermy virus protein 2b., Chen HY, Yang J, Lin C, Yuan YA, EMBO Rep. 2008 Aug;9(8):754-60. Epub 2008 Jul 4.PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18600235 18600235]<br>
*The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition., Lingel A, Simon B, Izaurralde E, Sattler M. EMBO Rep. 2005 Dec;6(12):1149-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16270100 16270100]<br>
*The structure of the flock house virus B2 protein, a viral suppressor of RNA interference, shows a novel mode of double-stranded RNA recognition., Lingel A, Simon B, Izaurralde E, Sattler M. EMBO Rep. 2005 Dec;6(12):1149-55. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16270100 16270100]<br>
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