5hnc: Difference between revisions
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==Synchrotron X-ray single crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels== | |||
<StructureSection load='5hnc' size='340' side='right'caption='[[5hnc]], [[Resolution|resolution]] 1.76Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5hnc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HNC FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hnc OCA], [https://pdbe.org/5hnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hnc RCSB], [https://www.ebi.ac.uk/pdbsum/5hnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hnc ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein microcrystals magnetically aligned in D2O hydrogels were subjected to neutron diffraction measurements, and reflections were observed for the first time to a resolution of 3.4 A from lysozyme microcrystals ( approximately 10 x 10 x 50 microm). This result demonstrated the possibility that magnetically oriented microcrystals consolidated in D2O gels may provide a promising means to obtain single-crystal neutron diffraction from proteins that do not crystallize at the sizes required for neutron diffraction structure determination. In addition, lysozyme microcrystals aligned in H2O hydrogels allowed structure determination at a resolution of 1.76 A at room temperature by X-ray diffraction. The use of gels has advantages since the microcrystals are measured under hydrated conditions. | |||
Neutron and X-ray single-crystal diffraction from protein microcrystals via magnetically oriented microcrystal arrays in gels.,Tsukui S, Kimura F, Kusaka K, Baba S, Mizuno N, Kimura T Acta Crystallogr D Struct Biol. 2016 Jul 1;72(Pt 7):823-9. doi:, 10.1107/S2059798316007415. Epub 2016 Jun 23. PMID:27377379<ref>PMID:27377379</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5hnc" style="background-color:#fffaf0;"></div> | ||
[[Category: Kimura | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Lysozyme 3D structures|Lysozyme 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Gallus gallus]] | |||
[[Category: Large Structures]] | |||
[[Category: Baba S]] | |||
[[Category: Kimura F]] | |||
[[Category: Kimura T]] | |||
[[Category: Kusaka K]] | |||
[[Category: Mizuno N]] | |||
[[Category: Tsukui S]] | |||
