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==Crystal structure of Thermus thermophilus Csm6==
==Crystal structure of Thermus thermophilus Csm6==
<StructureSection load='5fsh' size='340' side='right' caption='[[5fsh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='5fsh' size='340' side='right'caption='[[5fsh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5fsh]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FSH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FSH FirstGlance]. <br>
<table><tr><td colspan='2'>[[5fsh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FSH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FSH FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.301&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fsh OCA], [http://pdbe.org/5fsh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fsh RCSB], [http://www.ebi.ac.uk/pdbsum/5fsh PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fsh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fsh OCA], [https://pdbe.org/5fsh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fsh RCSB], [https://www.ebi.ac.uk/pdbsum/5fsh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fsh ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CSM6_THET8 CSM6_THET8] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). The type III-A Csm effector complex binds crRNA and acts as a crRNA-guided RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA cognate to the crRNA is required for all activities. This protein is not part of the Csm effector complex (Probable).  A single-strand-specific endoribonuclease (ssRNase) producing free 5'-OH (PubMed:26763118). Activity is approximately 1000-fold stimulated by cyclic oligoadenylate (cOA); only cyclic tetraadenylate (cA4) stimulates the ssRNase activity while linear oligoadenylates do not activate the RNase (PubMed:28663439). Another study showed stimulation by linear tetraadenylate at very high concentrations, but did not examine stimulation by cA4 (PubMed:28722012).<ref>PMID:26763118</ref> <ref>PMID:28663439</ref> <ref>PMID:28722012</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Jinek, M]]
[[Category: Large Structures]]
[[Category: Niewoehner, O]]
[[Category: Thermus thermophilus HB8]]
[[Category: Crispr-ca]]
[[Category: Jinek M]]
[[Category: Endonuclease]]
[[Category: Niewoehner O]]
[[Category: Hydrolase]]
[[Category: Ribonuclease]]
Retrieved from "https://proteopedia.org/5fsh"