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[[Image:1chk.gif|left|200px]]


{{Structure
==STREPTOMYCES N174 CHITOSANASE PH5.5 298K==
|PDB= 1chk |SIZE=350|CAPTION= <scene name='initialview01'>1chk</scene>, resolution 2.40&Aring;
<StructureSection load='1chk' size='340' side='right'caption='[[1chk]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
|SITE= <scene name='pdbsite=CAT:Putative+Catalytic+Residues'>CAT</scene>
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1chk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_sp._N174 Streptomyces sp. N174]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CHK FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
|GENE= CSN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1931 Streptomyces sp.])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1chk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1chk OCA], [https://pdbe.org/1chk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1chk RCSB], [https://www.ebi.ac.uk/pdbsum/1chk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1chk ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1chk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1chk OCA], [http://www.ebi.ac.uk/pdbsum/1chk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1chk RCSB]</span>
[https://www.uniprot.org/uniprot/CHIS_STRSN CHIS_STRSN] Aids in the defense against invading fungal pathogens by degrading their cell wall chitosan.
}}
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
'''STREPTOMYCES N174 CHITOSANASE PH5.5 298K'''
Check<jmol>
 
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ch/1chk_consurf.spt"</scriptWhenChecked>
==Overview==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
We report the 2.4 A X-ray crystal structure of a protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The structure was solved using phases acquired by SIRAS from a two-site methyl mercury derivative combined with solvent flattening and non-crystallographic two-fold symmetry averaging, and refined to an R-factor of 18.5%. The mostly alpha-helical fold reveals a structural core shared with several classes of lysozyme and barley endochitinase, in spite of a lack of shared sequence. Based on this structural similarity we postulate a putative active site, mechanism of action and mode of substrate recognition. It appears that Glu 22 acts as an acid and Asp 40 serves as a general base to activate a water molecule for an SN2 attack on the glycosidic bond. A series of amino-acid side chains and backbone carbonyl groups may bind the polycationic chitosan substrate in a deep electronegative binding cleft.
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==About this Structure==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1chk ConSurf].
1CHK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_sp. Streptomyces sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CHK OCA].  
<div style="clear:both"></div>
 
__TOC__
==Reference==
</StructureSection>
X-ray structure of an anti-fungal chitosanase from streptomyces N174., Marcotte EM, Monzingo AF, Ernst SR, Brzezinski R, Robertus JD, Nat Struct Biol. 1996 Feb;3(2):155-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8564542 8564542]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Streptomyces sp. N174]]
[[Category: Streptomyces sp.]]
[[Category: Marcotte EM]]
[[Category: Marcotte, E M.]]
[[Category: Robertus JD]]
[[Category: Robertus, J D.]]
[[Category: anti-fungal protein]]
[[Category: hydrolase]]
[[Category: o-glycosyl]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:21:46 2008''
Retrieved from "https://proteopedia.org/1chk"