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[[Image:1cpn.gif|left|200px]]


{{Structure
==NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS==
|PDB= 1cpn |SIZE=350|CAPTION= <scene name='initialview01'>1cpn</scene>, resolution 1.8&Aring;
<StructureSection load='1cpn' size='340' side='right'caption='[[1cpn]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
<table><tr><td colspan='2'>[[1cpn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CPN FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cpn OCA], [https://pdbe.org/1cpn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cpn RCSB], [https://www.ebi.ac.uk/pdbsum/1cpn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cpn ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1cpn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cpn OCA], [http://www.ebi.ac.uk/pdbsum/1cpn PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1cpn RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/GUB_PAEMA GUB_PAEMA]
 
== Evolutionary Conservation ==
'''NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cp/1cpn_consurf.spt"</scriptWhenChecked>
A jellyroll beta-sandwich protein, the Bacillus beta-glucanase H(A16-M), is used to probe the role of N-terminal peptide regions in protein folding in vivo. A gene encoding H(A16-M) is rearranged to place residues 1-58 of the protein behind a signal peptide and residues 59-214. The rearranged gene is expressed in Escherichia coli. The resultant circularly permuted protein, cpA16M-59, is secreted into the periplasm, correctly processed, and folded into a stable and active enzyme. Crystal structure analysis at 2.0-A resolution, R = 15.3%, shows cpA16M-59 to have a three-dimensional structure nearly identical with that of the parent beta-glucanase. An analogous experiment based on the wild-type Bacillus macerans beta-glucanase, giving rise to the circularly permuted variant cpMAC-57, yields the same results. Folding of these proteins, therefore, is not a vectorial process depending on the conformation adopted by their native N-terminal oligopeptides after ribosomal synthesis and translocation through the cytoplasmic membrane.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1CPN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paenibacillus_macerans Paenibacillus macerans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CPN OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cpn ConSurf].
 
<div style="clear:both"></div>
==Reference==
__TOC__
Native-like in vivo folding of a circularly permuted jellyroll protein shown by crystal structure analysis., Hahn M, Piotukh K, Borriss R, Heinemann U, Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10417-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7937966 7937966]
</StructureSection>
[[Category: Licheninase]]
[[Category: Large Structures]]
[[Category: Paenibacillus macerans]]
[[Category: Paenibacillus macerans]]
[[Category: Single protein]]
[[Category: Hahn M]]
[[Category: Hahn, M.]]
[[Category: Heinemann U]]
[[Category: Heinemann, U.]]
[[Category: hydrolase(glucanase)]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:26:09 2008''

Latest revision as of 15:41, 13 March 2024

NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS

1cpn, resolution 1.80Å

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