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| [[Image:1csl.jpg|left|200px]]
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| {{Structure
| | ==CRYSTAL STRUCTURE OF THE RRE HIGH AFFINITY SITE== |
| |PDB= 1csl |SIZE=350|CAPTION= <scene name='initialview01'>1csl</scene>, resolution 1.60Å
| | <StructureSection load='1csl' size='340' side='right'caption='[[1csl]], [[Resolution|resolution]] 1.60Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>
| | <table><tr><td colspan='2'>[[1csl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CSL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CSL FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1csl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1csl OCA], [https://pdbe.org/1csl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1csl RCSB], [https://www.ebi.ac.uk/pdbsum/1csl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1csl ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | __TOC__ |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1csl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1csl OCA], [http://www.ebi.ac.uk/pdbsum/1csl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1csl RCSB]</span>
| | </StructureSection> |
| }}
| | [[Category: Large Structures]] |
| | | [[Category: Ippolito JA]] |
| '''CRYSTAL STRUCTURE OF THE RRE HIGH AFFINITY SITE'''
| | [[Category: Steitz TA]] |
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| ==Overview==
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| The crystal structure of a 28 nt RNA fragment containing the human immunodeficiency virus type 1 (HIV-1) Rev response element high affinity binding site for Rev protein has been solved at 1.6 A resolution. The overall structure of the RRE helix is greatly distorted from A-form geometry by the presence of two purine-purine base-pairs and two single nucleotide bulges. G48 and G71 form a Hoogsteen-type asymmetric base-pair with G71 adopting a syn conformation. The non-canonical regions in the unliganded Rev response element molecule narrow the major groove width with respect to standard A-RNA. The Rev response element structure observed here represents a closed form of the Rev binding site and differs from conformations of the RNA observed previously by solution NMR studies.
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| ==About this Structure==
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| 1CSL is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CSL OCA].
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| ==Reference==
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| The structure of the HIV-1 RRE high affinity rev binding site at 1.6 A resolution., Ippolito JA, Steitz TA, J Mol Biol. 2000 Jan 28;295(4):711-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10656783 10656783]
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| [[Category: Protein complex]] | |
| [[Category: Ippolito, J A.]] | |
| [[Category: Steitz, T A.]] | |
| [[Category: hiv-1]]
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| [[Category: rre high affinity site]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:27:46 2008''
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