2vf7: Difference between revisions

Latest revision as of 09:59, 9 May 2024

Crystal structure of UvrA2 from Deinococcus radiodurans

Structural highlights

2vf7 is a 3 chain structure with sequence from Deinococcus radiodurans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	ADP, MG, ZN
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9RYW8_DEIRA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

UvrA proteins are key actors in DNA damage repair and play an essential role in prokaryotic nucleotide excision repair (NER), a pathway that is unique in its ability to remove a broad spectrum of DNA lesions. Understanding the DNA binding and damage recognition activities of the UvrA family is a critical component for establishing the molecular basis of this process. Here we report the structure of the class II UvrA2 from Deinococcus radiodurans in two crystal forms. These structures, coupled with mutational analyses and comparison with the crystal structure of class I UvrA from Bacillus stearothermophilus, suggest a previously unsuspected role for the identified insertion domains of UvrAs in both DNA binding and damage recognition. Taken together, the available information suggests a model for how UvrA interacts with DNA and thus sheds new light on the molecular mechanisms underlying the role of UvrA in the early steps of NER.

Structural and mutational analyses of Deinococcus radiodurans UvrA2 provide insight into DNA binding and damage recognition by UvrAs.,Timmins J, Gordon E, Caria S, Leonard G, Acajjaoui S, Kuo MS, Monchois V, McSweeney S Structure. 2009 Apr 15;17(4):547-58. PMID:19368888^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Timmins J, Gordon E, Caria S, Leonard G, Acajjaoui S, Kuo MS, Monchois V, McSweeney S. Structural and mutational analyses of Deinococcus radiodurans UvrA2 provide insight into DNA binding and damage recognition by UvrAs. Structure. 2009 Apr 15;17(4):547-58. PMID:19368888 doi:10.1016/j.str.2009.02.008

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OCA

[1] Timmins J, Gordon E, Caria S, Leonard G, Acajjaoui S, Kuo MS, Monchois V, McSweeney S. Structural and mutational analyses of Deinococcus radiodurans UvrA2 provide insight into DNA binding and damage recognition by UvrAs. Structure. 2009 Apr 15;17(4):547-58. PMID:19368888 doi:10.1016/j.str.2009.02.008

[1]

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF UVRA2 FROM DEINOCOCCUS RADIODURANS==
-<StructureSection load='2vf7' size='340' side='right' caption='[[2vf7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+==Crystal structure of UvrA2 from Deinococcus radiodurans==
+<StructureSection load='2vf7' size='340' side='right'caption='[[2vf7]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2vf7]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_radiodurans"_raj_et_al._1960 "micrococcus radiodurans" raj et al. 1960]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VF7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VF7 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2vf7]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VF7 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vf8|2vf8]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vf7 OCA], [http://pdbe.org/2vf7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vf7 RCSB], [http://www.ebi.ac.uk/pdbsum/2vf7 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vf7 OCA], [https://pdbe.org/2vf7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vf7 RCSB], [https://www.ebi.ac.uk/pdbsum/2vf7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vf7 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9RYW8_DEIRA Q9RYW8_DEIRA]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/2vf7_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vf/2vf7_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 30: / Line 33: @@
 __TOC__
 </StructureSection>
-[[Category: Micrococcus radiodurans raj et al. 1960]]
+[[Category: Deinococcus radiodurans]]
-[[Category: Caria, S]]
+[[Category: Large Structures]]
-[[Category: Gordon, E]]
+[[Category: Caria S]]
-[[Category: Kuo, M S]]
+[[Category: Gordon E]]
-[[Category: Leonard, G]]
+[[Category: Kuo MS]]
-[[Category: Mcsweeney, S]]
+[[Category: Leonard G]]
-[[Category: Monchois, V]]
+[[Category: McSweeney S]]
-[[Category: Timmins, J]]
+[[Category: Monchois V]]
-[[Category: Abc protein dna-binding protein]]
+[[Category: Timmins J]]
-[[Category: Atp-binding]]
-[[Category: Dna damage]]
-[[Category: Dna excision]]
-[[Category: Dna repair]]
-[[Category: Dna-binding]]
-[[Category: Dna-binding protein]]
-[[Category: Excision nuclease]]
-[[Category: Metal-binding]]
-[[Category: Nucleotide-binding]]
-[[Category: Sos response]]
-[[Category: Zinc-binding domain]]
-[[Category: Zinc-finger]]

2vf7: Difference between revisions

Latest revision as of 09:59, 9 May 2024

Crystal structure of UvrA2 from Deinococcus radiodurans

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

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