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| [[Image:1d64.gif|left|200px]]
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| {{Structure
| | ==CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES== |
| |PDB= 1d64 |SIZE=350|CAPTION= <scene name='initialview01'>1d64</scene>, resolution 2.100Å
| | <StructureSection load='1d64' size='340' side='right'caption='[[1d64]], [[Resolution|resolution]] 2.10Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PNT:1,3-BIS(4-AMIDINOPHENOXY)PENTANE'>PNT</scene>
| | <table><tr><td colspan='2'>[[1d64]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D64 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D64 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PNT:1,5-BIS(4-AMIDINOPHENOXY)PENTANE'>PNT</scene></td></tr> |
| |DOMAIN=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d64 OCA], [https://pdbe.org/1d64 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d64 RCSB], [https://www.ebi.ac.uk/pdbsum/1d64 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d64 ProSAT]</span></td></tr> |
| |RELATEDENTRY=
| | </table> |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1d64 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d64 OCA], [http://www.ebi.ac.uk/pdbsum/1d64 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1d64 RCSB]</span>
| | __TOC__ |
| }}
| | </StructureSection> |
| | | [[Category: Large Structures]] |
| '''CRYSTAL STRUCTURE OF A PENTAMIDINE-OLIGONUCLEOTIDE COMPLEX: IMPLICATIONS FOR DNA-BINDING PROPERTIES'''
| | [[Category: Edwards KJ]] |
| | | [[Category: Jenkins TC]] |
| | | [[Category: Neidle S]] |
| ==Overview==
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| The crystal structure of the complex formed between the dodecanucleotide d(CGCGAATTCGCG)2 and the drug pentamidine, which is active against the Pneumocystis carinii pathogen in AIDS patients, has been determined to a resolution of 2.1 A and an R-factor of 19.4%. Analysis of the structure has shown the drug to be bound in the 5'-AATT minor groove region of the duplex, with the amidinium groups H-bonded to adenine N3 atoms in an interstrand manner. The drug molecule adopts an extended conformation, and the immediate binding site spans four base pairs. Structural details of the drug-DNA interactions are discussed, and comparison is made with the dodecamer complex of the structurally similar berenil ligand.
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| ==About this Structure==
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| 1D64 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D64 OCA].
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| ==Reference==
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| Crystal structure of a pentamidine-oligonucleotide complex: implications for DNA-binding properties., Edwards KJ, Jenkins TC, Neidle S, Biochemistry. 1992 Aug 11;31(31):7104-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/1643044 1643044]
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| [[Category: Protein complex]] | |
| [[Category: Edwards, K J.]] | |
| [[Category: Jenkins, T C.]] | |
| [[Category: Neidle, S.]] | |
| [[Category: b-dna]]
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| [[Category: complexed with drug]]
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| [[Category: double helix]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 19:35:28 2008''
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