1ddp: Difference between revisions
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==Solution structure of a CISPLATIN-INDUCED [CATAGCTATG]2 Interstrand cross-link== | |||
<StructureSection load='1ddp' size='340' side='right'caption='[[1ddp]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1ddp]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DDP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DDP FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, models</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CPT:CISPLATIN'>CPT</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ddp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ddp OCA], [https://pdbe.org/1ddp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ddp RCSB], [https://www.ebi.ac.uk/pdbsum/1ddp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ddp ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
== | |||
The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin. | The widely used antitumor drug cis-diamminedichloroplatinum(II) (cisplatin or cis-DDP) reacts with DNA, cross-linking two purine residues through the N7 atoms, which reside in the major groove in B-form DNA. The solution structure of the short duplex [d(CAT-AGCTATG)]2 cross-linked at the GC:GC site was determined by nuclear magnetic resonance (NMR). The deoxyguanosine-bridging cis-diammineplatinum(II) lies in the minor groove, and the complementary deoxycytidines are extrahelical. The double helix is locally reversed to a left-handed form, and the helix is unwound and bent toward the minor groove. These findings were independently confirmed by results from a phase-sensitive gel electrophoresis bending assay. The NMR structure differs markedly from previously proposed models but accounts for the chemical reactivity, the unwinding, and the bending of cis-DDP interstrand cross-linked DNA and may be important in the formation and repair of these cross-links in chromatin. | ||
Solution structure of a cisplatin-induced DNA interstrand cross-link.,Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB Science. 1995 Dec 15;270(5243):1842-5. PMID:8525382<ref>PMID:8525382</ref> | |||
Solution structure of a cisplatin-induced DNA interstrand cross-link., Huang H, Zhu L, Reid BR, Drobny GP, Hopkins PB | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1ddp" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Drobny GP]] | |||
[[Category: Hopkins PB]] | |||
[[Category: Huang H]] | |||
[[Category: Reid BR]] | |||
[[Category: Zhu L]] | |||
Latest revision as of 05:42, 13 August 2026
Solution structure of a CISPLATIN-INDUCED [CATAGCTATG]2 Interstrand cross-link
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