3bwt: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
==Crystal structure of the RNA binding domain of Puf4 from Saccharomyces cerevisiae==
==Crystal structure of the RNA binding domain of Puf4 from Saccharomyces cerevisiae==
<StructureSection load='3bwt' size='340' side='right' caption='[[3bwt]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
<StructureSection load='3bwt' size='340' side='right'caption='[[3bwt]], [[Resolution|resolution]] 2.69&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3bwt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3BWT FirstGlance]. <br>
<table><tr><td colspan='2'>[[3bwt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BWT FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m6w|1m6w]], [[1m8x|1m8x]], [[1m8y|1m8y]], [[1m8z|1m8z]], [[1ib2|1ib2]], [[3bx2|3bx2]], [[3bx3|3bx3]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.69&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PUF4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bwt OCA], [https://pdbe.org/3bwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bwt RCSB], [https://www.ebi.ac.uk/pdbsum/3bwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bwt ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bwt OCA], [http://pdbe.org/3bwt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3bwt RCSB], [http://www.ebi.ac.uk/pdbsum/3bwt PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/PUF4_YEAST PUF4_YEAST]] Is not essential for haploid growth, but may affect diploid formation.  
[https://www.uniprot.org/uniprot/PUF4_YEAST PUF4_YEAST] Is not essential for haploid growth, but may affect diploid formation.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bwt_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bw/3bwt_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
Line 19: Line 19:
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bwt ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bwt ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Pumilio/FBF (PUF) family proteins are found in eukaryotic organisms and regulate gene expression post-transcriptionally by binding to sequences in the 3' untranslated region of target transcripts. PUF proteins contain an RNA binding domain that typically comprises eight alpha-helical repeats, each of which recognizes one RNA base. Some PUF proteins, including yeast Puf4p, have altered RNA binding specificity and use their eight repeats to bind to RNA sequences with nine or ten bases. Here we report the crystal structures of Puf4p alone and in complex with a 9-nucleotide (nt) target RNA sequence, revealing that Puf4p accommodates an 'extra' nucleotide by modest adaptations allowing one base to be turned away from the RNA binding surface. Using structural information and sequence comparisons, we created a mutant Puf4p protein that preferentially binds to an 8-nt target RNA sequence over a 9-nt sequence and restores binding of each protein repeat to one RNA base.
Basis of altered RNA-binding specificity by PUF proteins revealed by crystal structures of yeast Puf4p.,Miller MT, Higgin JJ, Hall TM Nat Struct Mol Biol. 2008 Apr;15(4):397-402. Epub 2008 Mar 9. PMID:18327269<ref>PMID:18327269</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3bwt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 18824]]
[[Category: Large Structures]]
[[Category: Hall, T M.T]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Higgin, J J]]
[[Category: Hall TMT]]
[[Category: Miller, M T]]
[[Category: Higgin JJ]]
[[Category: Ho endonuclease]]
[[Category: Miller MT]]
[[Category: Puf4]]
[[Category: Pumilio]]
[[Category: Rna binding]]
[[Category: Rna binding protein]]
[[Category: Transcription]]

Latest revision as of 09:31, 21 February 2024

Crystal structure of the RNA binding domain of Puf4 from Saccharomyces cerevisiae

3bwt, resolution 2.69Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=3bwt&oldid=4063625"