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| [[Image:1ev7.gif|left|200px]]
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| {{Structure
| | ==CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI== |
| |PDB= 1ev7 |SIZE=350|CAPTION= <scene name='initialview01'>1ev7</scene>, resolution 2.38Å
| | <StructureSection load='1ev7' size='340' side='right'caption='[[1ev7]], [[Resolution|resolution]] 2.38Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[1ev7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EV7 FirstGlance]. <br> |
| |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.38Å</td></tr> |
| |GENE= | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [https://pdbe.org/1ev7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB], [https://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ev7 ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ev7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ev7 OCA], [http://www.ebi.ac.uk/pdbsum/1ev7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ev7 RCSB]</span>
| | [https://www.uniprot.org/uniprot/T2N1_LENAE T2N1_LENAE] An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.<ref>PMID:7892605</ref> <ref>PMID:8932368</ref> <ref>PMID:12654995</ref> |
| }}
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| '''CRYSTAL STRUCTURE OF DNA RESTRICTION ENDONUCLEASE NAEI'''
| | ==See Also== |
| | | *[[Endonuclease 3D structures|Endonuclease 3D structures]] |
| | | == References == |
| ==Overview== | | <references/> |
| NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes.
| | __TOC__ |
| | | </StructureSection> |
| ==About this Structure==
| | [[Category: Large Structures]] |
| 1EV7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EV7 OCA].
| | [[Category: Lentzea aerocolonigenes]] |
| | | [[Category: Chen Y]] |
| ==Reference== | | [[Category: Colandene JD]] |
| Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase., Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H, EMBO J. 2000 Jun 15;19(12):3110-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10856254 10856254]
| | [[Category: Huai Q]] |
| [[Category: Lechevalieria aerocolonigenes]] | | [[Category: Luo F]] |
| [[Category: Single protein]] | | [[Category: Zhao Y]] |
| [[Category: Type II site-specific deoxyribonuclease]]
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| [[Category: Chen, Y.]] | |
| [[Category: Colandene, J D.]] | |
| [[Category: Huai, Q.]] | |
| [[Category: Luo, F.]] | |
| [[Category: Zhao, Y.]] | |
| [[Category: apo-naei]]
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| [[Category: cap]]
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| [[Category: helix-turn-helix]]
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| [[Category: restriction endonuclease]]
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| [[Category: topoisomerase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:10:15 2008''
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