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[[Image:1f5u.gif|left|200px]]


{{Structure
==SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA==
|PDB= 1f5u |SIZE=350|CAPTION= <scene name='initialview01'>1f5u</scene>
<StructureSection load='1f5u' size='340' side='right'caption='[[1f5u]]' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
<table><tr><td colspan='2'>[[1f5u]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1F5U FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
|GENE=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5u OCA], [https://pdbe.org/1f5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1f5u RCSB], [https://www.ebi.ac.uk/pdbsum/1f5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1f5u ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=
<div style="background-color:#fffaf0;">
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1f5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1f5u OCA], [http://www.ebi.ac.uk/pdbsum/1f5u PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1f5u RCSB]</span>
== Publication Abstract from PubMed ==
}}
 
'''SOLUTION STRUCTURE OF THE KISSING DIMER OF H3 GACG STEM-LOOP IN THE 5'-END DIMERIZATION SIGNAL OF MOLONEY MURINE LEUKEMIA VIRUS GENOMIC RNA'''
 
 
==Overview==
The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.
The dimerization of viral RNA through noncovalent interactions at their 5' ends is a key step in the life cycle of retroviruses. In Moloney murine leukemia virus, three stem-loops are important in this process. One is a self-complementary tetraloop (H1), but the other two stem-loops (H2, H3) contain highly conserved GACG tetraloops that are not self-complementary sequences. Using two-dimensional NMR, we determined the structure of the H3 stem-loop. Surprisingly, it forms a stable, homodimeric kissing complex through only two intermolecular G small middle dotC base pairs. Cross-strand interactions of the adenines adjacent to the intermolecular G small middle dotC base pairs, plus unusual strong electrostatic interactions around the base pairs, contribute to the unexpected stability. This structure shows how even stem-loops without self-complementary sequences can facilitate the intermolecular recognition between two identical RNAs, and thus initiate dimerization and encapsidation of retroviral RNAs.


==About this Structure==
A retroviral RNA kissing complex containing only two G.C base pairs.,Kim CH, Tinoco I Jr Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:10931958<ref>PMID:10931958</ref>
1F5U is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F5U OCA].
 
==Reference==
A retroviral RNA kissing complex containing only two G.C base pairs., Kim CH, Tinoco I Jr, Proc Natl Acad Sci U S A. 2000 Aug 15;97(17):9396-401. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10931958 10931958]
[[Category: Protein complex]]
[[Category: Jr., I Tinoco.]]
[[Category: Kim, C H.]]
[[Category: dimerization]]
[[Category: kissing dimer]]
[[Category: moloney murine leukemia virus]]
[[Category: nmr]]
[[Category: retrovirus]]
[[Category: stem-loop]]
[[Category: tetraloop]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 20:16:33 2008''
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1f5u" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Kim C-H]]
[[Category: Tinoco Jr I]]
Retrieved from "https://proteopedia.org/1f5u"