5i4t: Difference between revisions
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The | ==Immature hexagonal lattice of HIV-1 Gag== | ||
<StructureSection load='5i4t' size='340' side='right'caption='[[5i4t]], [[Resolution|resolution]] 3.27Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5i4t]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I4T FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.273Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4t OCA], [https://pdbe.org/5i4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i4t RCSB], [https://www.ebi.ac.uk/pdbsum/5i4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4t ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q72497_9HIV1 Q72497_9HIV1] Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex (By similarity). Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers (By similarity).[SAAS:SAAS012344_004_011858] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Virus assembly and maturation proceed through the programmed operation of molecular switches, which trigger both local and global structural rearrangements to produce infectious particles. HIV-1 contains an assembly and maturation switch that spans the C-terminal domain (CTD) of the capsid (CA) region and the first spacer peptide (SP1) of the precursor structural protein, Gag. The crystal structure of the CTD-SP1 Gag fragment is a goblet-shaped hexamer in which the cup comprises the CTD and an ensuing type II beta-turn, and the stem comprises a 6-helix bundle. The beta-turn is critical for immature virus assembly and the 6-helix bundle regulates proteolysis during maturation. This bipartite character explains why the SP1 spacer is a critical element of HIV-1 Gag but is not a universal property of retroviruses. Our results also indicate that HIV-1 maturation inhibitors suppress unfolding of the CA-SP1 junction and thereby delay access of the viral protease to its substrate. | |||
Crystal structure of an HIV assembly and maturation switch.,Wagner JM, Zadrozny KK, Chrustowicz J, Purdy MD, Yeager M, Ganser-Pornillos BK, Pornillos O Elife. 2016 Jul 14;5. pii: e17063. doi: 10.7554/eLife.17063. PMID:27416583<ref>PMID:27416583</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Ganser-Pornillos | <div class="pdbe-citations 5i4t" style="background-color:#fffaf0;"></div> | ||
[[Category: Pornillos | == References == | ||
[[Category: Wagner | <references/> | ||
[[Category: Zadrozny | __TOC__ | ||
</StructureSection> | |||
[[Category: Human immunodeficiency virus 1]] | |||
[[Category: Large Structures]] | |||
[[Category: Ganser-Pornillos BK]] | |||
[[Category: Pornillos O]] | |||
[[Category: Wagner JM]] | |||
[[Category: Zadrozny K]] | |||
