5ifg: Difference between revisions

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'''Unreleased structure'''


The entry 5ifg is ON HOLD
==Crystal structure of HigA-HigB complex from E. Coli==
<StructureSection load='5ifg' size='340' side='right'caption='[[5ifg]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ifg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IFG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.702&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ifg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ifg OCA], [https://pdbe.org/5ifg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ifg RCSB], [https://www.ebi.ac.uk/pdbsum/5ifg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ifg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HIGB_ECOLI HIGB_ECOLI] Toxic component of a toxin-antitoxin (TA) module. A probable translation-dependent mRNA interferase. Overexpression causes cessation of cell growth and inhibits cell proliferation via inhibition of translation; this blockage is overcome by subsequent expression of antitoxin HigA. Overexpression causes cleavage of a number of mRNAs in a translation-dependent fashion, suggesting this is an mRNA interferase.<ref>PMID:19943910</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The toxin-antitoxin system is ubiquitously existed in bacteria and archaea, performing a wide variety of functions modulating cell fitness in response to environmental cues. In this report, we solved the crystal structure of the toxin-antitoxin HigBA complex from E. coli K-12 to 2.7 A resolution. The crystal structure of the HigBA complex displays a hetero-tetramer (HigBA)2 form comprised by two HigB and two HigA subunits. Each toxin HigB resumes a microbial RNase T1 fold, characteristic of a three antiparallel beta-sheet core shielded by a few alpha-helices at either side. Each antitoxin HigA composed of all alpha-helices resembles a "C"-shaped clamp nicely encompassing a HigB in the (HigBA)2 complex. Two HigA monomers dimerize at their N-terminal domain. We showed that HigA helix alpha1 was essential for HigA dimerization and the hetero-tetramer (HigBA)2 formation, but not for a hetero-dimeric HigBA formation. HigA dimerization mediated by helix alpha1 was dispensable for DNA-binding, as a heterodimeric HigBA complex still bound to the higBA operator in vitro. The HigA C-terminal domain with a helix-turn-helix fold was essential for DNA binding. We also defined two palindromes in higBA operator specifically recognized by HigA and HigBA in vitro.


Authors: Yang, J.S., Zhou, K., Gao, z.Q., Liu, Q.S., Dong, Y.H.
Structural insight into the E. coli HigBA complex.,Yang J, Zhou K, Liu P, Dong Y, Gao Z, Zhang J, Liu Q Biochem Biophys Res Commun. 2016 Sep 30;478(4):1521-7. doi:, 10.1016/j.bbrc.2016.08.131. Epub 2016 Sep 4. PMID:27601326<ref>PMID:27601326</ref>


Description: crystal structure of HigA-HigB complex from E. Coli
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Gao, Z.Q]]
<div class="pdbe-citations 5ifg" style="background-color:#fffaf0;"></div>
[[Category: Zhou, K]]
== References ==
[[Category: Dong, Y.H]]
<references/>
[[Category: Yang, J.S]]
__TOC__
[[Category: Liu, Q.S]]
</StructureSection>
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Dong YH]]
[[Category: Gao zQ]]
[[Category: Liu QS]]
[[Category: Yang JS]]
[[Category: Zhou K]]
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