5i5i

Shewanella denitrificans nitrous oxide reductase, app form

spinqualitylabelsall models 5i5i, resolution 2.14Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5i5i is a 2 chain structure with sequence from Shewanella denitrificans OS217. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.14Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q12M27_SHEDO Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide.[HAMAP-Rule:MF_00716] Publication Abstract from PubMed The copper enzyme nitrous oxide reductase catalyzes the two-electron reduction of nitrous oxide (N2O) to dinitrogen (N2). Its maturation largely occurs in the periplasm and includes the insertion of at least one Ca2+ ion per monomer. Here we have investigated the role of this structural cation in recombinantly produced apo-N2OR from Shewanella denitrificans and have determined the three-dimensional structure of the protein by X-ray crystallography. In the absence of Ca2+, substantial parts of the enzyme surrounding the binding sites for the copper ions show structural disorder. Reconstitution of the binuclear CuA site was possible in vitro but required the presence of Ca2+ ions for a stable insertion of the center. In contrast, an excess of Ca2+ prevented copper insertion, and the structural analysis of the Ca2+ apo form revealed that the cation is sufficient to structure the disordered regions of the protein even in the absence of Cu ions, indicating that the geometry of the two noncanonical copper centers is largely predetermined by the protein structure. Role of Calcium in Secondary Structure Stabilization during Maturation of Nitrous Oxide Reductase.,Schneider LK, Einsle O Biochemistry. 2016 Feb 29. PMID:26885878 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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