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[[Image:1gtm.gif|left|200px]]


{{Structure
==STRUCTURE OF GLUTAMATE DEHYDROGENASE==
|PDB= 1gtm |SIZE=350|CAPTION= <scene name='initialview01'>1gtm</scene>, resolution 2.2&Aring;
<StructureSection load='1gtm' size='340' side='right'caption='[[1gtm]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
<table><tr><td colspan='2'>[[1gtm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GTM FirstGlance]. <br>
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_dehydrogenase_(NAD(P)(+)) Glutamate dehydrogenase (NAD(P)(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.1.3 1.4.1.3] </span>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gtm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gtm OCA], [https://pdbe.org/1gtm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gtm RCSB], [https://www.ebi.ac.uk/pdbsum/1gtm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gtm ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gtm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gtm OCA], [http://www.ebi.ac.uk/pdbsum/1gtm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1gtm RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/DHE3_PYRFU DHE3_PYRFU]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/1gtm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gtm ConSurf].
<div style="clear:both"></div>


'''STRUCTURE OF GLUTAMATE DEHYDROGENASE'''
==See Also==
 
*[[Glutamate dehydrogenase 3D structures|Glutamate dehydrogenase 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
BACKGROUND: The hyperthermophile Pyrococcus furiosus is one of the most thermostable organisms known, with an optimum growth temperature of 100 degrees C. The proteins from this organism display extreme thermostability. We have undertaken the structure determination of glutamate dehydrogenase from P. furiosus in order to gain further insights into the relationship between molecular structure and thermal stability. RESULTS: The structure of P. furiosus glutamate dehydrogenase, a homohexameric enzyme, has been determined at 2.2 A resolution and compared with the structure of glutamate dehydrogenase from the mesophile Clostridium symbiosum. CONCLUSIONS: Comparison of the structures of these two enzymes has revealed one major difference: the structure of the hyperthermophilic enzyme contains a striking series of ion-pair networks on the surface of the protein subunits and buried at both interdomain and intersubunit interfaces. We propose that the formation of such extended networks may represent a major stabilizing feature associated with the adaptation of enzymes to extreme temperatures.
[[Category: Large Structures]]
 
[[Category: Pyrococcus furiosus DSM 3638]]
==About this Structure==
[[Category: Britton KL]]
1GTM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GTM OCA].
[[Category: Pasquo A]]
 
[[Category: Rice DW]]
==Reference==
[[Category: Stillman TJ]]
The structure of Pyrococcus furiosus glutamate dehydrogenase reveals a key role for ion-pair networks in maintaining enzyme stability at extreme temperatures., Yip KS, Stillman TJ, Britton KL, Artymiuk PJ, Baker PJ, Sedelnikova SE, Engel PC, Pasquo A, Chiaraluce R, Consalvi V, Structure. 1995 Nov 15;3(11):1147-58. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8591026 8591026]
[[Category: Yip KSP]]
[[Category: Glutamate dehydrogenase (NAD(P)(+))]]
[[Category: Pyrococcus furiosus]]
[[Category: Single protein]]
[[Category: Britton, K L.]]
[[Category: Pasquo, A.]]
[[Category: Rice, D W.]]
[[Category: Stillman, T J.]]
[[Category: Yip, K S.P.]]
[[Category: nad]]
[[Category: nadp]]
[[Category: oxidoreductase]]
 
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Retrieved from "https://proteopedia.org/1gtm"