Kdo-8-phosphate synthase: Difference between revisions

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{{STRUCTURE_3fyp|  PDB=3fyp  | SIZE=400| SCENE= |right|CAPTION=Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]] }}
<StructureSection load='' size='350' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>
 
== Function ==
== Function ==
'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P).  '''Metallo KPS''' is a metal-requiring enzyme while '''Non-metallo KPS''' does not require a divalent cation for its activity.
'''Kdo-8-phosphate synthase''' or '''2-dehydro-3-deoxyphosphooctonate aldolase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P).  Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.
Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.


== Structural highlights ==
== Structural highlights ==
The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>.
The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/7'>homotetramer</scene>. The <scene name='52/525184/Cv/8'>active site of the metallo KPS contains a divalent cation and the PEP substrate</scene><ref>PMID:19447118</ref>. Water molecules shown as red spheres.
 
</StructureSection>
==3D structures of Kdo-8-phosphate synthase==
==3D structures of Kdo-8-phosphate synthase==
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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**[[1fxp]] - AaKPS + Cd – ''Aquifex aeolicus''<br />
**[[1fxp]] - AaKPS + Cd – ''Aquifex aeolicus''<br />
**[[1lrn]], [[1t99]] - AaKPS (mutant) + Cd<br />
**[[1lrn]], [[1t99]] - AaKPS (mutant) + Cd<br />
**[[1fx6]] - AaKPS <br />
**[[2nws]], [[2nx3]] - AaKPS (mutant) <br />
**[[2qkf]] – NmKPS + Na – ''Neisseria meningitides''<br />
**[[2qkf]] – NmKPS + Na – ''Neisseria meningitides''<br />
**[[3fyo]] - NmKPS (mutant) + Mn<br />
**[[3fyo]] - NmKPS (mutant) + Mn<br />
**[[3qpy]], [[3qpz]], [[3qq0]], [[3stc]], [[3ste]], [[3stf]], [[4jte]], [[4jtf]], [[4jtg]], [[4jth]], [[4jti]], [[4jtj]], [[4jtk]], [[4jtl]] - NmKPS (mutant) + Na<br />
**[[3qpy]], [[3qpz]], [[3qq0]], [[3stc]], [[3ste]], [[3stf]], [[4jte]], [[4jtf]], [[4jtg]], [[4jth]], [[4jti]], [[4jtj]], [[4jtk]], [[4jtl]] - NmKPS (mutant) + Na<br />
**[[3qq1]], [[3stg]] - NmKPS (mutant) <br />
**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />
**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />
**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />
**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />
**[[4z1a]] – HpKPS – ''Helicobacter pylori''<br />
**[[4z1b]] - HpKPS (mutant)<br />
**[[4z1c]] - HpKPS + Cd<br />
**[[6bng]] – KPS – ''Acinetobacter baumannii''<br />


*''Metallo Kdo8P binary complex''
*''Metallo Kdo8P binary complex''
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**[[1pck]] - AaKPS + PEP derivative + Cd<br />
**[[1pck]] - AaKPS + PEP derivative + Cd<br />
**[[1pe1]] - AaKPS + phosphoglyceric acid + Cd<br />
**[[1pe1]] - AaKPS + phosphoglyceric acid + Cd<br />
**[[2a21]] - EcKPS + PEP + Zn - ''Escherichia coli''<br />
**[[2a21]] - AaKPS + PEP + Zn <br />
**[[1jcx]], [[1pcw]] - AaKPS + inhibitor + Cd<br />
**[[1jcx]], [[1pcw]] - AaKPS + inhibitor + Cd<br />
**[[1fy6]] - AaKPS + A5P + Cd<br />
**[[1fy6]] - AaKPS + A5P + Cd<br />
**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />
**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />
**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />
**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />
**[[4z1d]] - HpKPS + PEP + Zn<br />
**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />
**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />
**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />
**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />
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**[[2a2i]] - AaKPS + A5P + PEP + Zn<br />
**[[2a2i]] - AaKPS + A5P + PEP + Zn<br />
**[[1lrq]], [[1t8x]] - AaKPS (mutant) + A5P + PEP + Cd<br />
**[[1lrq]], [[1t8x]] - AaKPS (mutant) + A5P + PEP + Cd<br />
**[[1fxq]] - AaKPS + A5P + PEP<br />
**[[2nx1]], [[2ef9]] - AaKPS (mutant) + A5P + PEP<br />
**[[2nxg]] - AaKPS (mutant) + octanoic acid derivative + A5P + PEP<br />
**[[3und]] - BpKPS + arabinose-5-phosphate + PEP<br />
**[[3und]] - BpKPS + arabinose-5-phosphate + PEP<br />


*Non-metallo Kdo8P
*Non-metallo Kdo8P


**[[1d9e]], [[1gg0]], [[1x8f]] – EcKPS <br />
**[[1d9e]], [[1gg0]], [[1x8f]], [[6u57]] – EcKPS - ''Escherichia coli''<br />
**[[1fx6]] - AaKPS <br />
**[[2nws]], [[2nx3]] - AaKPS (mutant) <br />
**[[1o60]] - KPS – ''Haemophilus influenza''<br />
**[[1o60]] - KPS – ''Haemophilus influenza''<br />
**[[3e9a]] - KPS – ''Vibrio cholerae''<br />
**[[3e9a]] - KPS – ''Vibrio cholerae''<br />
**[[3fs2]] - KPS – ''Brucella melitensis''<br />
**[[3fs2]] - KPS – ''Brucella melitensis''<br />
**[[3qq1]], [[3stg]] - NmKPS (mutant) <br />
**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''<br />
**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''
**[[6mdy]] – KPS – ''Legionella pneumophila''<br />


*''Non-metallo Kdo8P binary complex''
*''Non-metallo Kdo8P binary complex''


**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />
**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />
**[[1g7u]], [[1q3n]] - EcKPS + PEP<br />
**[[1g7u]], [[1q3n]] - EcKPS + PEP<br />
**[[1phq]], [[1pl9]] - EcKPS + PEP derivative<br />
**[[1phq]], [[1pl9]] - EcKPS + PEP derivative<br />
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**[[1x6u]] - EcKPS + Kdo-8-P
**[[1x6u]] - EcKPS + Kdo-8-P


*''Non-metallo Kdo8P ternary complex''
**[[1fxq]] - AaKPS + A5P + PEP<br />
**[[2nx1]], [[2ef9]] - AaKPS (mutant) + A5P + PEP<br />
**[[2nxg]] - AaKPS (mutant) + octanoic acid derivative + A5P + PEP<br />
}}
}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]
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