Kdo-8-phosphate synthase: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
Michal Harel (talk | contribs) No edit summary |
||
| (6 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
<StructureSection load=' | <StructureSection load='' size='350' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'> | ||
== Function == | == Function == | ||
'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>. | '''Kdo-8-phosphate synthase''' or '''2-dehydro-3-deoxyphosphooctonate aldolase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P). Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity. Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>. | ||
== Structural highlights == | == Structural highlights == | ||
The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>. | The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/7'>homotetramer</scene>. The <scene name='52/525184/Cv/8'>active site of the metallo KPS contains a divalent cation and the PEP substrate</scene><ref>PMID:19447118</ref>. Water molecules shown as red spheres. | ||
</StructureSection> | </StructureSection> | ||
==3D structures of Kdo-8-phosphate synthase== | ==3D structures of Kdo-8-phosphate synthase== | ||
| Line 23: | Line 23: | ||
**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br /> | **[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br /> | ||
**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br /> | **[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br /> | ||
**[[4z1a]] – HpKPS – ''Helicobacter pylori''<br /> | |||
**[[4z1b]] - HpKPS (mutant)<br /> | |||
**[[4z1c]] - HpKPS + Cd<br /> | |||
**[[6bng]] – KPS – ''Acinetobacter baumannii''<br /> | |||
*''Metallo Kdo8P binary complex'' | *''Metallo Kdo8P binary complex'' | ||
| Line 36: | Line 40: | ||
**[[3e12]] - AaKPS + Kdo-8-P + Cu<br /> | **[[3e12]] - AaKPS + Kdo-8-P + Cu<br /> | ||
**[[2nxi]], [[1fwn]] - AaKPS + PEP<br /> | **[[2nxi]], [[1fwn]] - AaKPS + PEP<br /> | ||
**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br /> | **[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br /> | ||
**[[4z1d]] - HpKPS + PEP + Zn<br /> | |||
**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br /> | **[[3fyp]] - NmKPS (mutant) + PEP + Mn<br /> | ||
**[[3tmq]] - BpKPS + arabinose-5-phosphate<br /> | **[[3tmq]] - BpKPS + arabinose-5-phosphate<br /> | ||
| Line 56: | Line 61: | ||
*Non-metallo Kdo8P | *Non-metallo Kdo8P | ||
**[[1d9e]], [[1gg0]], [[1x8f]] – EcKPS - ''Escherichia coli''<br /> | **[[1d9e]], [[1gg0]], [[1x8f]], [[6u57]] – EcKPS - ''Escherichia coli''<br /> | ||
**[[1o60]] - KPS – ''Haemophilus influenza''<br /> | **[[1o60]] - KPS – ''Haemophilus influenza''<br /> | ||
**[[3e9a]] - KPS – ''Vibrio cholerae''<br /> | **[[3e9a]] - KPS – ''Vibrio cholerae''<br /> | ||
**[[3fs2]] - KPS – ''Brucella melitensis''<br /> | **[[3fs2]] - KPS – ''Brucella melitensis''<br /> | ||
**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria'' | **[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''<br /> | ||
**[[6mdy]] – KPS – ''Legionella pneumophila''<br /> | |||
*''Non-metallo Kdo8P binary complex'' | *''Non-metallo Kdo8P binary complex'' | ||
Latest revision as of 08:35, 11 October 2021
| ||||||||||||
3D structures of Kdo-8-phosphate synthase
Updated on 11-October-2021
