Kdo-8-phosphate synthase: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
No edit summary
Michal Harel (talk | contribs)
32,850 edits
No edit summary
 
(5 intermediate revisions by 3 users not shown)
Line 1: Line 1:
<StructureSection load='3fyp' size='450' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>
<StructureSection load='' size='350' side='right' scene='52/525184/Cv/2' caption='Metallo Kdo-8-phosphate synthase tetramer complex with phosphoenolpyruvate, glycerol, Cl- (green), Na+ (large purple) and Mn+2 (small purple) ion, [[3fyp]]'>


== Function ==
== Function ==
'''Kdo-8-phosphate synthase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P).  Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity.  Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.
'''Kdo-8-phosphate synthase''' or '''2-dehydro-3-deoxyphosphooctonate aldolase''' (KPS) catalyzes the formation of the phosphorylated rare sugar Kdo-8-phosphate (Kdo-8-P) from phosphoenolpyruvate (PEP) and D-arabinose-5-phosphate (A5P).  Some bacteria like ''Aquifex aeolicus'' have '''Metallo KPS''' which is a metal-requiring enzyme while others like ''E.coli'' have a '''Non-metallo KPS''' which does not require a divalent cation for its activity.  Kdo-8-P is found in cell walls of higher plants and some green algae<ref>PMID:11904225</ref>.


== Structural highlights ==
== Structural highlights ==
The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/3'>homotetramer</scene>. The active site of the metallo KPS contains a divalent cation and the PEP substrate<ref>PMID:19447118</ref>.
The biological assembly of Metallo Kdo-8-phosphate synthase from ''Neisseria meningitidis'' is <scene name='52/525184/Cv/7'>homotetramer</scene>. The <scene name='52/525184/Cv/8'>active site of the metallo KPS contains a divalent cation and the PEP substrate</scene><ref>PMID:19447118</ref>. Water molecules shown as red spheres.
</StructureSection>
</StructureSection>
==3D structures of Kdo-8-phosphate synthase==
==3D structures of Kdo-8-phosphate synthase==
Line 23: Line 23:
**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />
**[[3sz8]] – BpKPS-2 – ''Burkholderia pseudomallei''<br />
**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />
**[[4lu0]] – KPS – ''Pseudomonas aeruginosa''<br />
**[[4z1a]] – HpKPS – ''Helicobacter pylori''<br />
**[[4z1b]] - HpKPS (mutant)<br />
**[[4z1c]] - HpKPS + Cd<br />
**[[6bng]] – KPS – ''Acinetobacter baumannii''<br />


*''Metallo Kdo8P binary complex''
*''Metallo Kdo8P binary complex''
Line 36: Line 40:
**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />
**[[3e12]] - AaKPS + Kdo-8-P + Cu<br />
**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />
**[[2nxi]], [[1fwn]] - AaKPS + PEP<br />
**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />  
**[[2nwr]], [[2nxh]] - AaKPS (mutant) + PEP<br />
**[[4z1d]] - HpKPS + PEP + Zn<br />  
**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />
**[[3fyp]] - NmKPS (mutant) + PEP + Mn<br />
**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />
**[[3tmq]] - BpKPS + arabinose-5-phosphate<br />
Line 56: Line 61:
*Non-metallo Kdo8P
*Non-metallo Kdo8P


**[[1d9e]], [[1gg0]], [[1x8f]] – EcKPS - ''Escherichia coli''<br />
**[[1d9e]], [[1gg0]], [[1x8f]], [[6u57]] – EcKPS - ''Escherichia coli''<br />
**[[1o60]] - KPS – ''Haemophilus influenza''<br />
**[[1o60]] - KPS – ''Haemophilus influenza''<br />
**[[3e9a]] - KPS – ''Vibrio cholerae''<br />
**[[3e9a]] - KPS – ''Vibrio cholerae''<br />
**[[3fs2]] - KPS – ''Brucella melitensis''<br />
**[[3fs2]] - KPS – ''Brucella melitensis''<br />
**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''
**[[3t4c]], [[3tml]] - KPS – ''Burkholderia ambifaria''<br />
**[[6mdy]] – KPS – ''Legionella pneumophila''<br />


*''Non-metallo Kdo8P binary complex''
*''Non-metallo Kdo8P binary complex''
Retrieved from "https://proteopedia.org/Kdo-8-phosphate_synthase"