3jc8

Architectural model of the type IVa pilus machine in a piliated state

Loading... Status: waiting for Mol* initialization Structural highlights 3jc8 is a 115 chain structure with sequence from Myxococcus xanthus DK 1622. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy Ligands: MEA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PILC_MYXXD Essential inner membrane component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers (By similarity). Controls both pilus assembly and disassembly and plays an important role in PilB localization to the complex and ATPase activity (PubMed:26851283).[UniProtKB:P22609] Publication Abstract from PubMed Type IVa pili are filamentous cell surface structures observed in many bacteria. They pull cells forward by extending, adhering to surfaces, and then retracting. We used cryo-electron tomography of intact Myxococcus xanthus cells to visualize type IVa pili and the protein machine that assembles and retracts them (the type IVa pilus machine, or T4PM) in situ, in both the piliated and nonpiliated states, at a resolution of 3 to 4 nanometers. We found that T4PM comprises an outer membrane pore, four interconnected ring structures in the periplasm and cytoplasm, a cytoplasmic disc and dome, and a periplasmic stem. By systematically imaging mutants lacking defined T4PM proteins or with individual proteins fused to tags, we mapped the locations of all 10 T4PM core components and the minor pilins, thereby providing insights into pilus assembly, structure, and function. Architecture of the type IVa pilus machine.,Chang YW, Rettberg LA, Treuner-Lange A, Iwasa J, Sogaard-Andersen L, Jensen GJ Science. 2016 Mar 11;351(6278):aad2001. doi: 10.1126/science.aad2001. Epub 2016, Mar 10. PMID:26965631 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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