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'''Unreleased structure'''


The entry 5j31 is ON HOLD  until Paper Publication
==Crystal structure of 14-3-3zeta in complex with an alkyne cross-linked cyclic peptide derived from ExoS==
<StructureSection load='5j31' size='340' side='right'caption='[[5j31]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5j31]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J31 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J31 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEZ:BENZOIC+ACID'>BEZ</scene>, <scene name='pdbligand=MK8:2-METHYL-L-NORLEUCINE'>MK8</scene>, <scene name='pdbligand=ZS8:(2S)-2-AMINO-2-METHYLDEC-8-YNOIC+ACID'>ZS8</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j31 OCA], [https://pdbe.org/5j31 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j31 RCSB], [https://www.ebi.ac.uk/pdbsum/5j31 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j31 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/1433Z_HUMAN 1433Z_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.<ref>PMID:9360956</ref> <ref>PMID:14578935</ref> <ref>PMID:15071501</ref> <ref>PMID:15644438</ref> <ref>PMID:16376338</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Macrocyclization can be used to constrain peptides in their bioactive conformations, thereby supporting target affinity and bioactivity. In particular, for the targeting of challenging protein-protein interactions, macrocyclic peptides have proven to be very useful. Available approaches focus on the stabilization of alpha-helices, which limits their general applicability. Here we report for the first time on the use of ring-closing alkyne metathesis for the stabilization of an irregular peptide secondary structure. A small library of alkyne-crosslinked peptides provided a number of derivatives with improved target affinity relative to the linear parent peptide. In addition, we report the crystal structure of the highest-affinity derivative in a complex with its protein target 14-3-3zeta. It can be expected that the alkyne-based macrocyclization of irregular binding epitopes should give rise to new scaffolds suitable for targeting of currently intractable proteins.


Authors: Wallraven, K., Cromm, P., Bier, D., Glas, A., Grossmann, T.
Constraining an Irregular Peptide Secondary Structure through Ring-Closing Alkyne Metathesis.,Cromm PM, Wallraven K, Glas A, Bier D, Furstner A, Ottmann C, Grossmann TN Chembiochem. 2016 Sep 6. doi: 10.1002/cbic.201600362. PMID:27596722<ref>PMID:27596722</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Wallraven, K]]
<div class="pdbe-citations 5j31" style="background-color:#fffaf0;"></div>
[[Category: Bier, D]]
 
[[Category: Cromm, P]]
==See Also==
[[Category: Grossmann, T]]
*[[14-3-3 protein 3D structures|14-3-3 protein 3D structures]]
[[Category: Glas, A]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Bier D]]
[[Category: Cromm P]]
[[Category: Glas A]]
[[Category: Grossmann T]]
[[Category: Wallraven K]]
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