5j5p: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: '''Unreleased structure''' The entry 5j5p is ON HOLD until Paper Publication Authors: Laponogov, I., Pan, X.-S., Skamrova, G., Umrekar, T., Fisher, L.M., Sanderson, M.R. Description: ...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 5j5p is ON HOLD  until Paper Publication
==AMP-PNP-stabilized ATPase domain of topoisomerase IV from Streptococcus pneumoniae, complex type I==
<StructureSection load='5j5p' size='340' side='right'caption='[[5j5p]], [[Resolution|resolution]] 1.97&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5j5p]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5J5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5J5P FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.97&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5j5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5j5p OCA], [https://pdbe.org/5j5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5j5p RCSB], [https://www.ebi.ac.uk/pdbsum/5j5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5j5p ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PARE_STRPN PARE_STRPN] Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule.<ref>PMID:17375187</ref> <ref>PMID:20596531</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type II topoisomerases alter DNA topology to control DNA supercoiling and chromosome segregation and are targets of clinically important anti-infective and anticancer therapeutics. They act as ATP-operated clamps to trap a DNA helix and transport it through a transient break in a second DNA. Here, we present the first X-ray crystal structure solved at 2.83 A of a closed clamp complete with trapped T-segment DNA obtained by co-crystallizing the ATPase domain of S. pneumoniae topoisomerase IV with a nonhydrolyzable ATP analogue and 14-mer duplex DNA. The ATPase dimer forms a 22 A protein hole occupied by the kinked DNA bound asymmetrically through positively charged residues lining the hole, and whose mutagenesis impacts the DNA decatenation, DNA relaxation and DNA-dependent ATPase activities of topo IV. These results and a side-bound DNA-ParE structure help explain how the T-segment DNA is captured and transported by a type II topoisomerase, and reveal a new enzyme-DNA interface for drug discovery.


Authors: Laponogov, I., Pan, X.-S., Skamrova, G., Umrekar, T., Fisher, L.M., Sanderson, M.R.
Trapping of the transport-segment DNA by the ATPase domains of a type II topoisomerase.,Laponogov I, Pan XS, Veselkov DA, Skamrova GB, Umrekar TR, Fisher LM, Sanderson MR Nat Commun. 2018 Jul 3;9(1):2579. doi: 10.1038/s41467-018-05005-x. PMID:29968711<ref>PMID:29968711</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Fisher, L.M]]
<div class="pdbe-citations 5j5p" style="background-color:#fffaf0;"></div>
[[Category: Laponogov, I]]
 
[[Category: Umrekar, T]]
==See Also==
[[Category: Skamrova, G]]
*[[Topoisomerase 3D structures|Topoisomerase 3D structures]]
[[Category: Pan, X.-S]]
== References ==
[[Category: Sanderson, M.R]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae]]
[[Category: Synthetic construct]]
[[Category: Fisher LM]]
[[Category: Laponogov I]]
[[Category: Pan X-S]]
[[Category: Sanderson MR]]
[[Category: Skamrova G]]
[[Category: Umrekar T]]
Retrieved from "https://proteopedia.org/5j5p"