5jj8: Difference between revisions
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New page: '''Unreleased structure''' The entry 5jj8 is ON HOLD Authors: Pinard, M.A., Kurian, J.J., Aggarwal, M., Agbandje-McKenna, M., McKenna, R. Description: [[Category: Unreleased Structure... |
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==Crystal Structure of the Beta Carbonic Anhydrase psCA3 isolated from Pseudomonas aeruginosa - alternate crystal packing form== | |||
<StructureSection load='5jj8' size='340' side='right'caption='[[5jj8]], [[Resolution|resolution]] 2.58Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5jj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JJ8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.585Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jj8 OCA], [https://pdbe.org/5jj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jj8 RCSB], [https://www.ebi.ac.uk/pdbsum/5jj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jj8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9HVB9_PSEAE Q9HVB9_PSEAE] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Cryoannealing has been demonstrated to improve the diffraction quality and resolution of crystals of the beta-carbonic anhydrase psCA3 concomitant with a change in space group. After initial flash-cooling in a liquid-nitrogen cryostream an X-ray diffraction data set from a psCA3 crystal was indexed in space group P21212 and was scaled to 2.6 A resolution, but subsequent cryoannealing studies revealed induced protein rearrangements in the crystal contacts, which transformed the space group to I222, with a corresponding improvement of 0.7 A in resolution. Although the change in diffraction resolution was significant, only minor changes in the psCA3 structure, which retained its catalytic `open' conformation, were observed. These findings demonstrate that cryoannealing can be successfully utilized to induce higher diffraction-quality crystals while maintaining enzymatically relevant conformations and may be useful as an experimental tool for structural studies of other enzymes where the initial diffraction quality is poor. | |||
Cryoannealing-induced space-group transition of crystals of the carbonic anhydrase psCA3.,Pinard MA, Kurian JJ, Aggarwal M, Agbandje-McKenna M, McKenna R Acta Crystallogr F Struct Biol Commun. 2016 Jul 1;72(Pt 7):573-7. doi:, 10.1107/S2053230X16009286. Epub 2016 Jun 28. PMID:27380376<ref>PMID:27380376</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5jj8" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Kurian | *[[Carbonic anhydrase 3D structures|Carbonic anhydrase 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Pseudomonas aeruginosa]] | |||
[[Category: Agbandje-McKenna M]] | |||
[[Category: Aggarwal M]] | |||
[[Category: Kurian JJ]] | |||
[[Category: McKenna R]] | |||
[[Category: Pinard MA]] | |||
Latest revision as of 18:54, 20 September 2023
Crystal Structure of the Beta Carbonic Anhydrase psCA3 isolated from Pseudomonas aeruginosa - alternate crystal packing form
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