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[[Image:1if0.gif|left|200px]]


{{Structure
==PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)==
|PDB= 1if0 |SIZE=350|CAPTION= <scene name='initialview01'>1if0</scene>, resolution 12.&Aring;
<SX load='1if0' size='340' side='right' viewer='molstar' caption='[[1if0]], [[Resolution|resolution]] 12.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[1if0]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_HK97 Escherichia virus HK97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IF0 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 12&#8491;</td></tr>
|GENE=  
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1if0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1if0 OCA], [https://pdbe.org/1if0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1if0 RCSB], [https://www.ebi.ac.uk/pdbsum/1if0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1if0 ProSAT]</span></td></tr>
|DOMAIN=
</table>
|RELATEDENTRY=[[1fh6|1FH6]]
== Function ==
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1if0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1if0 OCA], [http://www.ebi.ac.uk/pdbsum/1if0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1if0 RCSB]</span>
[https://www.uniprot.org/uniprot/CAPSD_BPHK7 CAPSD_BPHK7] Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.<ref>PMID:11000116</ref> <ref>PMID:21276801</ref> <ref>PMID:7669350</ref> <ref>PMID:7723020</ref>
}}
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
'''PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)'''
Check<jmol>
 
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/1if0_consurf.spt"</scriptWhenChecked>
==Overview==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
Large-scale conformational changes transform viral precursors into infectious virions. The structure of bacteriophage HK97 capsid, Head-II, was recently solved by crystallography, revealing a catenated cross-linked topology. We have visualized its precursor, Prohead-II, by cryoelectron microscopy and modeled the conformational change by appropriately adapting Head-II. Rigid-body rotations ( approximately 40 degrees) cause switching to an entirely different set of interactions; in addition, two motifs undergo refolding. These changes stabilize the capsid by increasing the surface area buried at interfaces and bringing the cross-link-forming residues, initially approximately 40 angstroms apart, close together. The inner surface of Prohead-II is negatively charged, suggesting that the transition is triggered electrostatically by DNA packaging.
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==About this Structure==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1if0 ConSurf].
1IF0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk97 Enterobacteria phage hk97]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IF0 OCA].  
<div style="clear:both"></div>
 
== References ==
==Reference==
<references/>
Virus maturation involving large subunit rotations and local refolding., Conway JF, Wikoff WR, Cheng N, Duda RL, Hendrix RW, Johnson JE, Steven AC, Science. 2001 Apr 27;292(5517):744-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11326105 11326105]
__TOC__
[[Category: Enterobacteria phage hk97]]
</SX>
[[Category: Single protein]]
[[Category: Escherichia virus HK97]]
[[Category: Cheng, N.]]
[[Category: Large Structures]]
[[Category: Conway, J F.]]
[[Category: Cheng N]]
[[Category: Duda, R L.]]
[[Category: Conway JF]]
[[Category: Hendrix, R W.]]
[[Category: Duda RL]]
[[Category: Johnson, J E.]]
[[Category: Hendrix RW]]
[[Category: Steven, A C.]]
[[Category: Johnson JE]]
[[Category: Wikoff, W R.]]
[[Category: Steven AC]]
[[Category: bacteriophage]]
[[Category: Wikoff WR]]
[[Category: capsid]]
[[Category: cryoem]]
[[Category: icosahedral virus]]
[[Category: pseudo-atomic model.]]
[[Category: virus]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 21:18:55 2008''

Latest revision as of 07:34, 7 February 2024

PSEUDO-ATOMIC MODEL OF BACTERIOPHAGE HK97 PROCAPSID (PROHEAD II)

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