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[[Image:1igv.gif|left|200px]]


{{Structure
==BOVINE CALBINDIN D9K BINDING MN2+==
|PDB= 1igv |SIZE=350|CAPTION= <scene name='initialview01'>1igv</scene>, resolution 1.85&Aring;
<StructureSection load='1igv' size='340' side='right'caption='[[1igv]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
|SITE= <scene name='pdbsite=MSE:Mn+Binding+Ef-Hand+Loop'>MSE</scene>
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>
<table><tr><td colspan='2'>[[1igv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IGV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IGV FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
|GENE= synthetic gene ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1igv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1igv OCA], [https://pdbe.org/1igv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1igv RCSB], [https://www.ebi.ac.uk/pdbsum/1igv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1igv ProSAT]</span></td></tr>
|RELATEDENTRY=[[3icb|3icb]], [[4icb|4icb]], [[1ig5|1IG5]], [[1clb|1clb]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1igv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1igv OCA], [http://www.ebi.ac.uk/pdbsum/1igv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1igv RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/S100G_BOVIN S100G_BOVIN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/1igv_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1igv ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional structures of the magnesium- and manganese-bound forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly identical but deviate significantly from both the calcium bound form and the metal ion-free (apo) form. The largest structural differences are seen in the C-terminal EF-hand, and involve changes in both metal ion coordination and helix packing. The N-terminal calcium binding site is not occupied by any metal ion in the magnesium and manganese structures, and shows little structural deviation from the apo and calcium bound forms. 1H-NMR and UV spectroscopic studies at physiological ion concentrations show that the C-terminal site of the protein is significantly populated by magnesium at resting cell calcium levels, and that there is a negative allosteric interaction between magnesium and calcium binding. Calcium binding was found to occur with positive cooperativity at physiological magnesium concentration.


'''BOVINE CALBINDIN D9K BINDING MN2+'''
Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.,Andersson M, Malmendal A, Linse S, Ivarsson I, Forsen S, Svensson LA Protein Sci. 1997 Jun;6(6):1139-47. PMID:9194174<ref>PMID:9194174</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 1igv" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The three-dimensional structures of the magnesium- and manganese-bound forms of calbindin D9k were determined to 1.6 A and 1.9 A resolution, respectively, using X-ray crystallography. These two structures are nearly identical but deviate significantly from both the calcium bound form and the metal ion-free (apo) form. The largest structural differences are seen in the C-terminal EF-hand, and involve changes in both metal ion coordination and helix packing. The N-terminal calcium binding site is not occupied by any metal ion in the magnesium and manganese structures, and shows little structural deviation from the apo and calcium bound forms. 1H-NMR and UV spectroscopic studies at physiological ion concentrations show that the C-terminal site of the protein is significantly populated by magnesium at resting cell calcium levels, and that there is a negative allosteric interaction between magnesium and calcium binding. Calcium binding was found to occur with positive cooperativity at physiological magnesium concentration.
*[[S100 proteins 3D structures|S100 proteins 3D structures]]
 
== References ==
==About this Structure==
<references/>
1IGV is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry 6ICB. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IGV OCA].
__TOC__
 
</StructureSection>
==Reference==
Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k., Andersson M, Malmendal A, Linse S, Ivarsson I, Forsen S, Svensson LA, Protein Sci. 1997 Jun;6(6):1139-47. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9194174 9194174]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Andersson, E M.]]
[[Category: Andersson EM]]
[[Category: calcium-binding protein]]
[[Category: ef-hand]]
[[Category: manganese binding]]
 
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Retrieved from "https://proteopedia.org/1igv"