5jpq: Difference between revisions
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The | ==Cryo-EM structure of the 90S pre-ribosome== | ||
<SX load='5jpq' size='340' side='right' viewer='molstar' caption='[[5jpq]], [[Resolution|resolution]] 7.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5jpq]] is a 27 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum Chaetomium thermophilum] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JPQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JPQ FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 7.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jpq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jpq OCA], [https://pdbe.org/5jpq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jpq RCSB], [https://www.ebi.ac.uk/pdbsum/5jpq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jpq ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G0S5L1_CHATD G0S5L1_CHATD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The 90S pre-ribosome is an early biogenesis intermediate formed during co-transcriptional ribosome formation, composed of approximately 70 assembly factors and several small nucleolar RNAs (snoRNAs) that associate with nascent pre-rRNA. We report the cryo-EM structure of the Chaetomium thermophilum 90S pre-ribosome, revealing how a network of biogenesis factors including 19 beta-propellers and large alpha-solenoid proteins engulfs the pre-rRNA. Within the 90S pre-ribosome, we identify the UTP-A, UTP-B, Mpp10-Imp3-Imp4, Bms1-Rcl1, and U3 snoRNP modules, which are organized around 5'-ETS and partially folded 18S rRNA. The U3 snoRNP is strategically positioned at the center of the 90S particle to perform its multiple tasks during pre-rRNA folding and processing. The architecture of the elusive 90S pre-ribosome gives unprecedented structural insight into the early steps of pre-rRNA maturation. Nascent rRNA that is co-transcriptionally folded and given a particular shape by encapsulation within a dedicated mold-like structure is reminiscent of how polypeptides use chaperone chambers for their protein folding. | |||
Architecture of the 90S Pre-ribosome: A Structural View on the Birth of the Eukaryotic Ribosome.,Kornprobst M, Turk M, Kellner N, Cheng J, Flemming D, Kos-Braun I, Kos M, Thoms M, Berninghausen O, Beckmann R, Hurt E Cell. 2016 Jul 14;166(2):380-93. doi: 10.1016/j.cell.2016.06.014. PMID:27419870<ref>PMID:27419870</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[ | </div> | ||
[[Category: | <div class="pdbe-citations 5jpq" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Beckmann | *[[Ribosome 3D structures|Ribosome 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: Flemming | __TOC__ | ||
[[Category: | </SX> | ||
[[Category: Kornprobst | [[Category: Chaetomium thermophilum]] | ||
[[Category: Kos-Braun | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae S288C]] | |||
[[Category: Beckmann R]] | |||
[[Category: Berninghausen O]] | |||
[[Category: Cheng J]] | |||
[[Category: Flemming D]] | |||
[[Category: Hurt E]] | |||
[[Category: Kornprobst M]] | |||
[[Category: Kos M]] | |||
[[Category: Kos-Braun IC]] | |||
[[Category: Thoms M]] | |||
[[Category: Turk M]] | |||
Latest revision as of 19:01, 20 September 2023
Cryo-EM structure of the 90S pre-ribosome
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