5b60: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 5b60 is ON HOLD Authors: Jin, Shengyang., Sun, Jian., Wunder, Tobias., Tang, Desong., Mueller-Caja, Oliver Martin., Gao, Yonggui. Description: Carb... |
No edit summary |
||
| (6 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Crystal structure of PtLCIB4 S47R mutant, a homolog of the limiting CO2-inducible protein LCIB== | ||
<StructureSection load='5b60' size='340' side='right'caption='[[5b60]], [[Resolution|resolution]] 2.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5b60]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaeodactylum_tricornutum Phaeodactylum tricornutum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5B60 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5B60 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5b60 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5b60 OCA], [https://pdbe.org/5b60 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5b60 RCSB], [https://www.ebi.ac.uk/pdbsum/5b60 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5b60 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B7FR29_PHATC B7FR29_PHATC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Aquatic microalgae have evolved diverse CO2-concentrating mechanisms (CCMs) to saturate the carboxylase with its substrate, to compensate for the slow kinetics and competing oxygenation reaction of the key photosynthetic CO2-fixing enzyme rubisco. The limiting CO2-inducible B protein (LCIB) is known to be essential for CCM function in Chlamydomonas reinhardtii To assign a function to this previously uncharacterized protein family, we purified and characterized a phylogenetically diverse set of LCIB homologs. Three of the six homologs are functional carbonic anhydrases (CAs). We determined the crystal structures of LCIB and limiting CO2-inducible C protein (LCIC) from C. reinhardtii and a CA-functional homolog from Phaeodactylum tricornutum, all of which harbor motifs bearing close resemblance to the active site of canonical beta-CAs. Our results identify the LCIB family as a previously unidentified group of beta-CAs, and provide a biochemical foundation for their function in the microalgal CCMs. | |||
Structural insights into the LCIB protein family reveals a new group of beta-carbonic anhydrases.,Jin S, Sun J, Wunder T, Tang D, Cousins AB, Sze SK, Mueller-Cajar O, Gao YG Proc Natl Acad Sci U S A. 2016 Dec 20;113(51):14716-14721. doi:, 10.1073/pnas.1616294113. Epub 2016 Dec 1. PMID:27911826<ref>PMID:27911826</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5b60" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: Wunder | [[Category: Large Structures]] | ||
[[Category: Phaeodactylum tricornutum]] | |||
[[Category: Gao Y]] | |||
[[Category: Jin S]] | |||
[[Category: Mueller-Caja OM]] | |||
[[Category: Sun J]] | |||
[[Category: Tang D]] | |||
[[Category: Wunder T]] | |||
