5g4t: Difference between revisions
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New page: '''Unreleased structure''' The entry 5g4t is ON HOLD until Paper Publication Authors: Huang, L., Lilley, D.M.J. Description: The structure of a quasi-cyclic six k-turn duplex RNA speci... |
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The | ==The structure of a quasi-cyclic six k-turn duplex RNA species== | ||
<StructureSection load='5g4t' size='340' side='right'caption='[[5g4t]], [[Resolution|resolution]] 2.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5g4t]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Haloarcula_marismortui Haloarcula marismortui]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5G4T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5G4T FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.751Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5g4t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5g4t OCA], [https://pdbe.org/5g4t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5g4t RCSB], [https://www.ebi.ac.uk/pdbsum/5g4t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5g4t ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
k-Turns are widespread RNA architectural elements that mediate tertiary interactions. We describe a double-kink-turn motif comprising two inverted k-turns that forms a tight horse-shoe structure that can assemble into a variety of shapes by coaxial association of helical ends. Using X-ray crystallography we show that these assemble with two (dumbell), three (triangle) and four units (square), with or without bound protein, within the crystal lattice. In addition, exchange of a single basepair can almost double the pore radius or shape of a molecular assembly. On the basis of this analysis we synthesized a 114 nt self-complementary RNA containing six k-turns. The crystal structure of this species shows that it forms a quasi-cyclic triangular object. These are randomly disposed about the three-fold axis in the crystal lattice, generating a circular RNA of quasi D3 symmetry with a shape reminiscent of that of a cyclohexane molecule in its chair conformation. This work demonstrates that the k-turn is a powerful building block in the construction of nano-scale molecular objects, and illustrates why k-turns are widely used in natural RNA molecules to organize long-range architecture and mediate tertiary contacts. | |||
A quasi-cyclic RNA nano-scale molecular object constructed using kink turns.,Huang L, Lilley DM Nanoscale. 2016 Aug 18;8(33):15189-95. doi: 10.1039/c6nr05186c. PMID:27506301<ref>PMID:27506301</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5g4t" style="background-color:#fffaf0;"></div> | ||
[[Category: Huang | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Haloarcula marismortui]] | |||
[[Category: Large Structures]] | |||
[[Category: Huang L]] | |||
[[Category: Lilley DMJ]] | |||
Latest revision as of 13:38, 26 July 2023
The structure of a quasi-cyclic six k-turn duplex RNA species
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