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New page: '''Unreleased structure''' The entry 5jus is ON HOLD Authors: Abeyrathne, P., Koh, C.S., Grant, T., Grigorieff, N., Korostelev, A.A. Description: Saccharomyces cerevisiae 80S ribosome ...
 
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'''Unreleased structure'''


The entry 5jus is ON HOLD
==Saccharomyces cerevisiae 80S ribosome bound with elongation factor eEF2-GDP-sordarin and Taura Syndrome Virus IRES, Structure III (mid-rotated 40S subunit)==
<SX load='5jus' size='340' side='right' viewer='molstar' caption='[[5jus]], [[Resolution|resolution]] 4.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5jus]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JUS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JUS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DDE:{3-[4-(2-AMINO-2-CARBOXY-ETHYL)-1H-IMIDAZOL-2-YL]-1-CARBAMOYL-PROPYL}-TRIMETHYL-AMMONIUM'>DDE</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO1:[1R-(1.ALPHA.,3A.BETA.,4.BETA.,4A.BETA.,7.BETA.,7A.ALPHA.,8A.BETA.)]8A-[(6-DEOXY-4-O-METHYL-BETA-D-ALTROPYRANOSYLOXY)METHYL]-4-FORMYL-4,4A,5,6,7,7A,8,8A-OCTAHYDRO-7-METHYL-3-(1-METHYLETHYL)-1,4-METHANO-S-INDACENE-3A(1H)-CARBOXYLIC+ACID'>SO1</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5jus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jus OCA], [https://pdbe.org/5jus PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5jus RCSB], [https://www.ebi.ac.uk/pdbsum/5jus PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5jus ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL5_YEAST RL5_YEAST] Binds 5S RNA and is required for 60S subunit assembly.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Internal ribosome entry sites (IRESs) mediate cap-independent translation of viral mRNAs. Using electron cryo-microscopy of a single specimen, we present five ribosome structures formed with the Taura syndrome virus IRES and translocase eEF2*GTP bound with sordarin. The structures suggest a trajectory of IRES translocation, required for translation initiation, and provide an unprecedented view of eEF2 dynamics. The IRES rearranges from extended to bent to extended conformations. This inchworm-like movement is coupled with ribosomal inter-subunit rotation and 40S head swivel. eEF2, attached to the 60S subunit, slides along the rotating 40S subunit to enter the A site. Its diphthamide-bearing tip at domain IV separates the tRNA-mRNA-like pseudoknot I (PKI) of the IRES from the decoding center. This unlocks 40S domains, facilitating head swivel and biasing IRES translocation via hitherto-elusive intermediates with PKI captured between the A and P sites. The structures suggest missing links in our understanding of tRNA translocation.


Authors: Abeyrathne, P., Koh, C.S., Grant, T., Grigorieff, N., Korostelev, A.A.
Ensemble cryo-EM uncovers inchworm-like translocation of a viral IRES through the ribosome.,Abeyrathne PD, Koh CS, Grant T, Grigorieff N, Korostelev AA Elife. 2016 May 9;5. pii: e14874. doi: 10.7554/eLife.14874. PMID:27159452<ref>PMID:27159452</ref>


Description: Saccharomyces cerevisiae 80S ribosome bound with elongation factor eEF2-GDP-sordarin and Taura Syndrome Virus IRES, Structure III (mid-rotated 40S subunit)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Korostelev, A.A]]
<div class="pdbe-citations 5jus" style="background-color:#fffaf0;"></div>
[[Category: Grigorieff, N]]
 
[[Category: Koh, C.S]]
==See Also==
[[Category: Grant, T]]
*[[Elongation factor 3D structures|Elongation factor 3D structures]]
[[Category: Abeyrathne, P]]
*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
== References ==
<references/>
__TOC__
</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Abeyrathne P]]
[[Category: Grant T]]
[[Category: Grigorieff N]]
[[Category: Koh CS]]
[[Category: Korostelev AA]]
Retrieved from "https://proteopedia.org/5jus"