1ixi: Difference between revisions

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[[Image:1ixi.gif|left|200px]]


{{Structure
==PHOSPHATE-BINDING PROTEIN MUTANT WITH ASP 56 REPLACED BY ASN COMPLEX WITH MONOBASIC PHOSPHATE ION==
|PDB= 1ixi |SIZE=350|CAPTION= <scene name='initialview01'>1ixi</scene>, resolution 1.89&Aring;
<StructureSection load='1ixi' size='340' side='right'caption='[[1ixi]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene>
<table><tr><td colspan='2'>[[1ixi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IXI FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
|GENE= POTENTIAL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2HP:DIHYDROGENPHOSPHATE+ION'>2HP</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ixi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ixi OCA], [https://pdbe.org/1ixi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ixi RCSB], [https://www.ebi.ac.uk/pdbsum/1ixi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ixi ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ixi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ixi OCA], [http://www.ebi.ac.uk/pdbsum/1ixi PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1ixi RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/PSTS_ECOLI PSTS_ECOLI] Part of the ABC transporter complex PstSACB involved in phosphate import.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ixi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ixi ConSurf].
<div style="clear:both"></div>


'''PHOSPHATE-BINDING PROTEIN MUTANT WITH ASP 56 REPLACED BY ASN COMPLEX WITH MONOBASIC PHOSPHATE ION'''
==See Also==
 
*[[Phosphate-binding protein|Phosphate-binding protein]]
 
__TOC__
==Overview==
</StructureSection>
A very short hydrogen bond between an Asp and a phosphate is established in two high resolution structures (0.98 and 1.05 A). A mutant complex that changes the Asp to an Asn, which forms a normal hydrogen bond, has a similar free energy of binding to the wild type complex, suggesting that the contribution of the short hydrogen bond is not extraordinarily strong.
 
==About this Structure==
1IXI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXI OCA].
 
==Reference==
A low energy short hydrogen bond in very high resolution structures of protein receptor--phosphate complexes., Wang Z, Luecke H, Yao N, Quiocho FA, Nat Struct Biol. 1997 Jul;4(7):519-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9228942 9228942]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Quiocho, F A.]]
[[Category: Quiocho FA]]
[[Category: Wang, Z.]]
[[Category: Wang Z]]
[[Category: binding protein]]
[[Category: mutant]]
[[Category: phosphate transport]]
[[Category: phosphate-binding]]
 
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Retrieved from "https://proteopedia.org/1ixi"