4o62

CW-type zinc finger of ZCWPW2 in complex with the amino terminus of histone H3

spinqualitylabelsall models 4o62, resolution 1.78Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4o62 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.78Å Ligands: M3L, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function H33_HUMAN Publication Abstract from PubMed Covalent modifications of histone N-terminal tails play a critical role in regulating chromatin structure and controlling gene expression. These modifications are controlled by histone-modifying enzymes and read out by histone-binding proteins. Numerous proteins have been identified as histone modification readers. Here we report the family-wide characterization of histone binding abilities of human CW domain-containing proteins. We demonstrate that the CW domains in ZCWPW2 and MORC3/4 selectively recognize histone H3 trimethylated at Lys-4, similar to ZCWPW1 reported previously, while the MORC1/2 and LSD2 lack histone H3 Lys-4 binding ability. Our crystal structures of the CW domains of ZCWPW2 and MORC3 in complex with the histone H3 trimethylated at Lys-4 peptide reveal the molecular basis of this interaction. In each complex, two tryptophan residues in the CW domain form the "floor" and "right wall," respectively, of the methyllysine recognition cage. Our mutation results based on ZCWPW2 reveal that the right wall tryptophan residue is essential for binding, and the floor tryptophan residue enhances binding affinity. Our structural and mutational analysis highlights the conserved roles of the cage residues of CW domain across the histone methyllysine binders but also suggests why some CW domains lack histone binding ability. Family-wide Characterization of Histone Binding Abilities of Human CW Domain-containing Proteins.,Liu Y, Tempel W, Zhang Q, Liang X, Loppnau P, Qin S, Min J J Biol Chem. 2016 Apr 22;291(17):9000-13. doi: 10.1074/jbc.M116.718973. Epub 2016, Mar 1. PMID:26933034 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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