5k83: Difference between revisions
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New page: '''Unreleased structure''' The entry 5k83 is ON HOLD Authors: Xiao, X., Li, S.-X., Yang, H., Chen, X.S. Description: Crystal Structure of a Primate APOBEC3G N-Domain, in Complex with s... |
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The | ==Crystal Structure of a Primate APOBEC3G N-Domain, in Complex with ssDNA== | ||
<StructureSection load='5k83' size='340' side='right'caption='[[5k83]], [[Resolution|resolution]] 2.39Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5k83]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5K83 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5K83 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.39Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5k83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5k83 OCA], [https://pdbe.org/5k83 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5k83 RCSB], [https://www.ebi.ac.uk/pdbsum/5k83 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5k83 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/M1GSK9_MACMU M1GSK9_MACMU] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
APOBEC3G (A3G) is a potent restriction factor of HIV-1. The N-terminal domain of A3G (A3G-CD1) is responsible for oligomerization and nucleic acid binding, both of which are essential for anti-HIV activity. As a countermeasure, HIV-1 viral infectivity factor (Vif) binds A3G-CD1 to mediate A3G degradation. The structural basis for the functions of A3G-CD1 remains elusive. Here, we report the crystal structures of a primate A3G-CD1 (rA3G-CD1) alone and in complex with single-stranded DNA (ssDNA). rA3G-CD1 shares a conserved core structure with the previously determined catalytic APOBECs, but displays unique features for surface charge, dimerization and nucleic acid binding. Its co-crystal structure with ssDNA reveals how the conformations of loops and residues surrounding the Zn-coordinated centre (Zn-centre) change upon DNA binding. The dimerization interface of rA3G-CD1 is important for oligomerization, nucleic acid binding and Vif-mediated degradation. These findings elucidate the molecular basis of antiviral mechanism and HIV-Vif targeting of A3G. | |||
Crystal structures of APOBEC3G N-domain alone and its complex with DNA.,Xiao X, Li SX, Yang H, Chen XS Nat Commun. 2016 Aug 2;7:12193. doi: 10.1038/ncomms12193. PMID:27480941<ref>PMID:27480941</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5k83" style="background-color:#fffaf0;"></div> | ||
[[Category: Li | == References == | ||
[[Category: Xiao | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Macaca mulatta]] | |||
[[Category: Chen XS]] | |||
[[Category: Li S-X]] | |||
[[Category: Xiao X]] | |||
[[Category: Yang H]] | |||
