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[[Image:1jax.gif|left|200px]]


{{Structure
==Structure of Coenzyme F420H2:NADP+ Oxidoreductase (FNO)==
|PDB= 1jax |SIZE=350|CAPTION= <scene name='initialview01'>1jax</scene>, resolution 1.80&Aring;
<StructureSection load='1jax' size='340' side='right'caption='[[1jax]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
<table><tr><td colspan='2'>[[1jax]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JAX FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
|GENE= AF0892 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2234 Archaeoglobus fulgidus])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jax OCA], [https://pdbe.org/1jax PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jax RCSB], [https://www.ebi.ac.uk/pdbsum/1jax PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jax ProSAT]</span></td></tr>
|RELATEDENTRY=[[1jay|1JAY]]
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1jax FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jax OCA], [http://www.ebi.ac.uk/pdbsum/1jax PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1jax RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/FNO_ARCFU FNO_ARCFU] Catalyzes the reversible reduction of NADP(+) by F420H(2). In this reaction the proS hydrogen at C5 of F420 is transferred into the proS position at C4 of NADPH.<ref>PMID:11726492</ref> [REFERENCE:2]
 
== Evolutionary Conservation ==
'''Structure of Coenzyme F420H2:NADP+ Oxidoreductase (FNO)'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ja/1jax_consurf.spt"</scriptWhenChecked>
Cofactor F420 is a 5'-deazaflavin derivative first discovered in methanogenic archaea but later found also to be present in some bacteria. As a coenzyme, it is involved in hydride transfer reactions and as a prosthetic group in the DNA photolyase reaction. We report here for the first time on the crystal structure of an F420-dependent oxidoreductase bound with F420. The structure of F420H2:NADP+ oxidoreductase resolved to 1.65 A contains two domains: an N-terminal domain characteristic of a dinucleotide-binding Rossmann fold and a smaller C-terminal domain. The nicotinamide and the deazaflavin part of the two coenzymes are bound in the cleft between the domains such that the Si-faces of both face each other at a distance of 3.1 A, which is optimal for hydride transfer. Comparison of the structures bound with and without substrates reveals that of the two substrates NADP has to bind first, the binding being associated with an induced fit.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1JAX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JAX OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jax ConSurf].
 
<div style="clear:both"></div>
==Reference==
== References ==
Structures of F420H2:NADP+ oxidoreductase with and without its substrates bound., Warkentin E, Mamat B, Sordel-Klippert M, Wicke M, Thauer RK, Iwata M, Iwata S, Ermler U, Shima S, EMBO J. 2001 Dec 3;20(23):6561-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11726492 11726492]
<references/>
__TOC__
</StructureSection>
[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Ermler, U.]]
[[Category: Ermler U]]
[[Category: Mamat, B.]]
[[Category: Mamat B]]
[[Category: Shima, S.]]
[[Category: Shima S]]
[[Category: Thauer, R.]]
[[Category: Thauer R]]
[[Category: Warkentin, E.]]
[[Category: Warkentin E]]
[[Category: rossman fold]]
 
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Retrieved from "https://proteopedia.org/1jax"