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| {{STRUCTURE_3e9l| PDB=3e9l | SIZE=400| SCENE= |right|CAPTION=Human pre-mRNA-splicing factor 8 endonuclease domain complex with Na+ (purple) and Cl- (green) ions, [[3e9l]] }}
| | <StructureSection load='' size='350' side='right' caption='Yeast pre-mRNA-splicing factor 8 (green) complex with AAR2 (cyan) (PDB entry [[3zef]])' scene='46/462200/Cv/1'> |
| | __TOC__ |
| == Function == | | == Function == |
| '''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing<ref>PMID:10394358</ref>. | | '''Pre-mRNA-splicing factors (Prp)''' are the proteins which associate with mRNA at its various splicing sites to produce alternative splicing<ref>PMID:10394358</ref>. |
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| *'''Prp18''' and '''Bud31''' are essential for the catalytic step in pre-mRNA splicing process.<br /> | | *'''Prp18''' and '''Bud31''' are essential for the catalytic step in pre-mRNA splicing process.<br /> |
| *'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br /> | | *'''snRNA-associated-splicing factor 24''' binds preferentially to the U4/U6 hybrid snRNAs. For details see [[Prp24]]<br /> |
| *[[Prp19]]<br /> | | *For '''Prp19''' see [[Prp19]]<br /> |
| *[[Prp40]].<br /> | | *For '''Prp20''' see [[Srp20-Human Alternative Splicing Factor]]<br /> |
| | *'''Prp31''' functions in forming the U4/U5-U6 tri-snRNP.<br /> |
| | *For '''Prp40''' see [[Prp40]].<br /> |
| *'''Prpcwc2''' is a yeast splicing factor which plays a role in the generation of a functional splicing center '''Prp RBM22''' is the human homolog of Prpcwc2.<br /> | | *'''Prpcwc2''' is a yeast splicing factor which plays a role in the generation of a functional splicing center '''Prp RBM22''' is the human homolog of Prpcwc2.<br /> |
| *'''Prpcwc26''' or '''Bud13''' belongs to the pre-mRNA retention and splicing complex RES.<br /> | | *'''Prpcwc26''' or '''Bud13''' belongs to the pre-mRNA retention and splicing complex RES.<br /> |
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| == 3D Structures of pre-mRNA splicing factors == | | == Disease == |
| | Mutations in Prp8 and Prp31 cause retinitis pigmentosa<ref>PMID:11468273</ref>. |
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| Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
| | == Structural highlights == |
| {{#tree:id=OrganizedByTopic|openlevels=0|
| | <scene name='46/462200/Cv/3'>Prp8 is a multi-domain protein containing an RNA-binding domain and a Jab1/MPN domain</scene>. |
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| *Pre-mRNA-splicing factor 2
| | == 3D Structures of pre-mRNA splicing factors == |
| | | [[Pre-mRNA splicing factors 3D structures]] |
| **[[3beg]] – hPrp P33 subunit + Ser/Thr-protein kinase – human<BR />
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| **[[2o3d]] – hPrp P33 subunit
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| *Pre-mRNA-splicing factor 3
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| **[[1x4q]] – hPrp PWI domain - NMR<BR />
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| *Pre-mRNA-splicing factor 3B
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| **[[3lqv]], [[2f9d]], [[2f9j]] – hPrp residues 377-415 + pre-mRNA branch site protein 14<BR />
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| **[[2fho]] - hPrp residues 379-424 + spliceosomal protein P14 - NMR<BR />
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| **[[2do5]] – hPrp subunit 2 – NMR<BR />
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| **[[1x5t]] - hPrp subunit 4 – NMR<BR />
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| **[[1x5u]] - hPrp subunit 4 RNA-binding domain – NMR<BR />
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| *Pre-mRNA-splicing factor 8
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| **[[3sbt]], [[4ilh]] – yPrp RNAseH like domain + A1 cistron-splicing factor AAR2 – yeast<BR />
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| **[[4jk7]], [[4jk9]] - yPrp RNAseH like domain <br />
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| **[[4ilj]], [[4jk8]], [[4jka]], [[4jkb]], [[4jkc]], [[4jkd]], [[4jke]], [[4jkf]], [[4jkg]], [[4jkh]] - yPrp RNAseH like domain (mutant)<br />
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| **[[3e66]] – yPrp β-finger domain<BR />
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| **[[3e9o]], [[3e9p]] - yPrp RNA-binding domain<BR />
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| **[[2p87]], [[2og4]], [[3sbg]] - yPrp C terminal<BR />
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| **[[3zef]], [[4i43]] – yPrp + A1 cistron-splicing factor AAR2<br />
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| **[[4bgd]] – yPrp JAB1/MPN domain + pre-mRNA-splicing helicase BRR2<br />
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| **[[4kit]] – yPrp JAB1/MPN domain + U5 small nuclear ribonucleoprotein helicase <br />
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| **[[4ilg]] – yPrp JAB1/MPN+RNAseH domains + A1 cistron-splicing factor AAR2 <br />
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| **[[4bgd]] – yPrp JAB1/MPN+RNAseH domains + A1 cistron-splicing factor AAR2 + pre-mRNA-splicing helicase BRR2<br />
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| **[[3lru]] – hPrp residues 1831-1990<BR />
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| **[[3e9l]] – hPrp endonuclease domain<BR />
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| **[[3enb]] - hPrp core domain IV<BR />
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| **[[2p8r]] – Prp C terminal (mutant) – ''Caenorhabditis elegans''
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| *Pre-mRNA-splicing factor 9
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| **[[4dgw]] – yPrp + Prp 21 + Prp 11
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| *Pre-mRNA-splicing factor 11
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| **[[4dgw]] – yPrp + Prp 21 + Prp 9
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| *Pre-mRNA-splicing factor 18
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| **[[2dk4]] – hPrp splicing factor motif domain - NMR<BR />
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| *Pre-mRNA-splicing factor 19
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| **[[3lrv]] – yPrp WD40 domain<BR />
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| **[[2bay]] – yPrp U-box<BR />
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| **[[1n87]] - yPrp U-box - NMR<BR />
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| *Pre-mRNA-splicing factor 20
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| **[[2i2y]] – Prp RNA-binding domain + RNA – ''Streptococcus'' – NMR<BR />
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| **[[2i38]] - hPrp RNA-binding domain (mutant) – NMR<BR />
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| *Pre-mRNA-splicing factor 21
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| **[[4dgw]] – yPrp + Prp 9 + Prp 11
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| *snRNA-associated-splicing factor 24
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| **[[2l9w]] – yPrp C terminal – NMR<BR />
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| **[[2kh9]] – yPrp residues 115-197 + RNA – NMR<BR />
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| **[[2go9]] – yPrp RNA-binding domains – NMR<BR />
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| **[[2ghp]] - yPrp RNA-binding domains
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| *Pre-mRNA-splicing factor 31
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| **[[2ozb]] – hPrp + RNA + tri-snRNP <br />
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| **[[3siu]], [[3siv]] – hPrp + NHP2-like protein + snRNP <br />
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| *Pre-mRNA-splicing factor 40
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| **[[1o6w]] – yPrp WW domain – yeast - NMR<br />
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| **[[2b7e]] – yPrp FF1 domain - NMR<br />
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| **[[2kfd]] – yPrp FF4 domain - NMR<br />
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| *Pre-mRNA-splicing factor 43P
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| **[[2xau]], [[3kx2]] – yPrp + ADP
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| *Pre-mRNA-splicing factor cwc2
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| **[[3tp2]], [[3u1l]], [[3u1m]] – yPrp <br />
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| *Pre-mRNA-splicing factor cwc26
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| **[[2mkc]] – yPrp + U2 SNRNP component IST3 + Pre-mRNA leakage protein<br />
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| **[[4uqt]] – yPrp + U2 SNRNP component IST3 <br />
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| *Pre-mRNA-splicing factor bud31
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| **[[2my1]] – yPrp - NMR<br />
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| *Pre-mRNA-splicing factor sad1
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| **[[4msx]] – yPrp <br />
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| *Pre-mRNA-splicing factor RBM22
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| **[[2ytc]] – yPrp RNA-binding domain - NMR<BR />
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| *Pre-mRNA-splicing factor RDS3
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| **[[2k0a]] – yPrp - NMR<BR />
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| *Pre-mRNA-splicing factor URN1
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| **[[2juc]] – yPrp FF domain - NMR<BR />
| | </StructureSection> |
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| == References == | | == References == |
