Proline utilization A: Difference between revisions

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== Function ==

'''Proline utilization A (PutA)''' is a bifunctional flavoprotein which acts as a transcriptional repressor of the put regulon or as a membrane-bound enzyme which catalyzes the oxidation of proline to glutamate. PutA domains include the DNA-binding domain (PRODH), the FAD-dependent proline dehydrogenase domain and the D-pyrroline-5-carboxylate dehydrogenase domain. The binding of proline to the PRODH active site and subsequent reduction of FAD causes a conformation change in PutA and enhance its membrane affinity. As a membrane-bound protein PutA switches from its repressor activity to its enzymatic role<ref>PMID:19994913</ref>.

'''Proline utilization A (PutA)''' or '''bifunctional protein PutA''' is a bifunctional flavoprotein which acts as a transcriptional repressor of the put regulon or as a membrane-bound enzyme which catalyzes the oxidation of proline to glutamate. PutA domains include the DNA-binding domain (PRODH), the FAD-dependent proline dehydrogenase domain and the D-pyrroline-5-carboxylate dehydrogenase domain. The binding of proline to the PRODH active site and subsequent reduction of FAD causes a conformation change in PutA and enhance its membrane affinity. As a membrane-bound protein PutA switches from its repressor activity to its enzymatic role<ref>PMID:19994913</ref>.

== Structural highlights ==

The active site residue Tyr540 helps in substrate preference for proline over hydroxyproline. The [[3e2q]] structure displayed here contains the Tyr540Ser mutant<ref>PMID:19140736</ref>.

The <scene name='70/706260/Cv/5'>active site residue Tyr540 helps in substrate preference for proline over hydroxyproline</scene>. Water molecules are shown as red spheres. The [[3e2q]] structure displayed here contains the <scene name='70/706260/Cv/6'>Tyr540Ser mutant</scene><ref>PMID:19140736</ref>.

</StructureSection>

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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}

{{#tree:id=OrganizedByTopic|openlevels=0|

*PutA

[[4nm9]] – GsPutA + FAD – ''Geobacter sulfurreducens''

**[[4nm9]] – GsPutA + FAD – ''Geobacter sulfurreducens''

[[4nma]] – GsPutA + FAD + tetrahydrofuran derivative

**[[7na0]] – GsPutA (mutant) + FAD

[[4nmb]] – GsPutA + FAD + lactate

**[[4nma]] – GsPutA + FAD + tetrahydrofuran derivative

[[4nmc]] – GsPutA + FAD + zwittergent

**[[2gpe]] – EcPutA DNA-binding domain

[[4nmd]] – GsPutA + FAD + dithionite

**[[2ay0]] – EcPutA DNA-binding domain (mutant)

[[4nme]] – GsPutA + FAD + propargylglycine

**[[4q71]], [[4q72]], [[4q73]] – BdPutA (mutant) + FAD – ''Bradyrhizobium diazoefficiens''

[[4nmf]] – GsPutA + FAD derivative + menadione bisulfite

**[[4h6r]] – DrPutA + FAD – ''Deinococcus radiodurans''

[[1tiw]] – EcPutA PRODH domain + FAD + tetrahydrofuran derivative ~~– ''Escherichia coli''~~

**[[2gpe]] - EcPutA DNA-binding domain - ''Escherichia coli''

[[3e2r]], [[4jny]], [[4jnz]] – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative

**[[2jxg]], [[2jxh]] - PpPutA DNA-binding domain - ''Pseudomonas putida'' - NMR

[[1tj0]], [[1tj1]] – EcPutA PRODH domain + FAD + lactate

**[[6ufp]], [[6vz9]] – SmPutA (mutant) + FAD – ''Sinorhizobium melioli''

[[1tj2]] – EcPutA PRODH domain + FAD + acetate

[[2fzn]] – EcPutA PRODH domain + FAD + proline

*PutA complex

[[3e2q]], [[3e2s]] – EcPutA PRODH domain (mutant) + FAD + proline derivative

[[2fzm]] – EcPutA PRODH domain + FAD + SO2

**[[4nmb]] – GsPutA + FAD + lactate

[[3itg]] – EcPutA PRODH domain + FAD + propargylglycine

**[[4nmc]] – GsPutA + FAD + zwittergent

[[~~2gpe~~]] – EcPutA DNA-binding domain

**[[4nmd]] – GsPutA + FAD + dithionite

[[~~2ay0~~]] – EcPutA DNA-binding ~~domain (mutant)~~

**[[4nme]] – GsPutA + FAD + propargylglycine

[[~~2rbf~~]] – ~~EcPutA~~ DNA-binding domain + DNA

**[[4nmf]] – GsPutA + FAD derivative + menadione bisulfite

[[~~4o8a~~]] – ~~EcPutA DNA-binding~~ + ~~PRODH domains~~ + FAD

**[[1tiw]] – EcPutA PRODH domain + FAD + tetrahydrofuran derivative

**[[3e2r]], [[4jny]], [[4jnz]] – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative

**[[1tj0]], [[1tj1]] – EcPutA PRODH domain + FAD + lactate

**[[1tj2]] – EcPutA PRODH domain + FAD + acetate

**[[2fzn]] – EcPutA PRODH domain + FAD + proline

**[[3e2q]], [[3e2s]] – EcPutA PRODH domain (mutant) + FAD + proline derivative

**[[2fzm]] – EcPutA PRODH domain + FAD + SO2

**[[3itg]] – EcPutA PRODH domain + FAD + propargylglycine

**[[2rbf]] – EcPutA DNA-binding domain + DNA

**[[4o8a]] – EcPutA DNA-binding + PRODH domains + FAD

**[[4h6q]] – DrPutA + FAD + THF

**[[3haz]] - PutA + FAD + NAD - ''Bradyrhyzobium japonicum''

**[[5ux5]] - PutA + FAD + NAD - ''Corynebacterium freiburgense''

**[[5kf6]], [[5kf7]], [[6ufp]], [[6vz9]] - PutA + FAD + NAD + THF - ''Sinorhizobium meliloti''

**[[2jxi]] - PpPutA DNA-binding domain + DNA

**[[5ur2]] - PutA + FAD derivative + inhibitor - ''Bdellovibrio bacteriovorus''

**[[6x9a]], [[6x9b]], [[6x9c]], [[6x9d]] – SmPutA + FAD + proline derivative

**[[6x99]], [[9bbo]] – SmPutA + FAD + Glu derivative

**[[9e5w]] – SmPutA + NAD + propargylglycine

**[[9c8c]] – SmPutA + NAD + FAD + allylgylglycine

**[[9c35]], [[9c36]] – SmPutA + NAD + FAD + Glu derivative

**[[9c34]] – SmPutA + NAD + FAD + Pro

**[[9dl8]], [[9dl9]], [[9e0a]], [[9e0b]], [[9e0c]], [[9e0d]], [[9e0e]] – SmPutA + NAD + FAD + alcohol

**[[9dl7]] – SmPutA + NAD + FAD + urea derivative

**[[9dl2]], [[9dl3]], [[9dl6]] – SmPutA + NAD + FAD + carboxylic acid derivative

**[[9dl4]] – SmPutA + NAD + FAD + pyridinethiol

**[[9dl5]] – SmPutA + NAD + FAD + indanone derivative

**[[7my9]], [[7mya]], [[7myb]], [[7myc]] – SmPutA + FAD + dithiolane

**[[6bsn]] – BdPutA + FAD + proline

}}

== References ==

[[Category:Topic Page]]

@@ Line 1: / Line 1: @@
-<StructureSection load='3e2q' size='340' side='right' caption='E. coli PutA proline dehydrogenase domain with cofactor FAD complex with hydroxyproline (PDB code [[3e2q]])' scene=''>
+<StructureSection load='' size='350' side='right' caption='E. coli PutA proline dehydrogenase domain with cofactor FAD complex with hydroxyproline (PDB code [[3e2q]])' scene='70/706260/Cv/1'>
 == Function ==
-'''Proline utilization A (PutA)''' is a bifunctional flavoprotein which acts as a transcriptional repressor of the put regulon or as a membrane-bound enzyme which catalyzes the oxidation of proline to glutamate. PutA domains include the DNA-binding domain (PRODH), the FAD-dependent proline dehydrogenase domain and the D-pyrroline-5-carboxylate dehydrogenase domain.  The binding of proline to the PRODH active site and subsequent reduction of FAD causes a conformation change in PutA and enhance its membrane affinity.  As a membrane-bound protein PutA switches from its repressor activity to its enzymatic role<ref>PMID:19994913</ref>.
+'''Proline utilization A (PutA)''' or '''bifunctional protein PutA''' is a bifunctional flavoprotein which acts as a transcriptional repressor of the put regulon or as a membrane-bound enzyme which catalyzes the oxidation of proline to glutamate. PutA domains include the DNA-binding domain (PRODH), the FAD-dependent proline dehydrogenase domain and the D-pyrroline-5-carboxylate dehydrogenase domain.  The binding of proline to the PRODH active site and subsequent reduction of FAD causes a conformation change in PutA and enhance its membrane affinity.  As a membrane-bound protein PutA switches from its repressor activity to its enzymatic role<ref>PMID:19994913</ref>.
 == Structural highlights ==
-The active site residue Tyr540 helps in substrate preference for proline over hydroxyproline.  The [[3e2q]] structure displayed here contains the Tyr540Ser mutant<ref>PMID:19140736</ref>.
+The <scene name='70/706260/Cv/5'>active site residue Tyr540 helps in substrate preference for proline over hydroxyproline</scene>. Water molecules are shown as red spheres. The [[3e2q]] structure displayed here contains the <scene name='70/706260/Cv/6'>Tyr540Ser mutant</scene><ref>PMID:19140736</ref>.
 </StructureSection>
@@ Line 10: / Line 10: @@
 Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
+{{#tree:id=OrganizedByTopic|openlevels=0|
+*PutA
-[[4nm9]] – GsPutA + FAD – ''Geobacter sulfurreducens'' <br />
+**[[4nm9]] – GsPutA + FAD – ''Geobacter sulfurreducens'' <br />
-[[4nma]] – GsPutA + FAD + tetrahydrofuran derivative <br />
+**[[7na0]] – GsPutA (mutant) + FAD <br />
-[[4nmb]] – GsPutA + FAD + lactate <br />
+**[[4nma]] – GsPutA + FAD + tetrahydrofuran derivative <br />
-[[4nmc]] – GsPutA + FAD + zwittergent <br />
+**[[2gpe]] – EcPutA DNA-binding domain <br />
-[[4nmd]] – GsPutA + FAD + dithionite <br />
+**[[2ay0]] – EcPutA DNA-binding domain (mutant)<br />
-[[4nme]] – GsPutA + FAD + propargylglycine <br />
+**[[4q71]], [[4q72]], [[4q73]] – BdPutA (mutant) + FAD – ''Bradyrhizobium diazoefficiens''<br />
-[[4nmf]] – GsPutA + FAD derivative + menadione bisulfite<br />
+**[[4h6r]] – DrPutA + FAD – ''Deinococcus radiodurans''<br />
-[[1tiw]] – EcPutA PRODH domain + FAD + tetrahydrofuran derivative – ''Escherichia coli''<br />
+**[[2gpe]] - EcPutA DNA-binding domain - ''Escherichia coli''<br />
-[[3e2r]], [[4jny]], [[4jnz]] – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative <br />
+**[[2jxg]], [[2jxh]] - PpPutA DNA-binding domain - ''Pseudomonas putida'' - NMR<br />
-[[1tj0]], [[1tj1]] – EcPutA PRODH domain + FAD + lactate<br />
+**[[6ufp]], [[6vz9]] – SmPutA (mutant) + FAD – ''Sinorhizobium melioli''<br />
-[[1tj2]] – EcPutA PRODH domain + FAD + acetate<br />
-[[2fzn]] – EcPutA PRODH domain + FAD + proline<br />
+*PutA complex
-[[3e2q]], [[3e2s]] – EcPutA PRODH domain (mutant) + FAD + proline derivative<br />
-[[2fzm]] – EcPutA PRODH domain + FAD + SO2<br />
+**[[4nmb]] – GsPutA + FAD + lactate <br />
-[[3itg]] – EcPutA PRODH domain + FAD + propargylglycine <br />
+**[[4nmc]] – GsPutA + FAD + zwittergent <br />
-[[2gpe]] – EcPutA DNA-binding domain <br />
+**[[4nmd]] – GsPutA + FAD + dithionite <br />
-[[2ay0]] – EcPutA DNA-binding domain (mutant)<br />
+**[[4nme]] – GsPutA + FAD + propargylglycine <br />
-[[2rbf]] – EcPutA DNA-binding domain + DNA<br />
+**[[4nmf]] – GsPutA + FAD derivative + menadione bisulfite<br />
-[[4o8a]] – EcPutA DNA-binding + PRODH domains + FAD <br />
+**[[1tiw]] – EcPutA PRODH domain + FAD + tetrahydrofuran derivative <br />
+**[[3e2r]], [[4jny]], [[4jnz]] – EcPutA PRODH domain (mutant) + FAD + tetrahydrofuran derivative <br />
+**[[1tj0]], [[1tj1]] – EcPutA PRODH domain + FAD + lactate<br />
+**[[1tj2]] – EcPutA PRODH domain + FAD + acetate<br />
+**[[2fzn]] – EcPutA PRODH domain + FAD + proline<br />
+**[[3e2q]], [[3e2s]] – EcPutA PRODH domain (mutant) + FAD + proline derivative<br />
+**[[2fzm]] – EcPutA PRODH domain + FAD + SO2<br />
+**[[3itg]] – EcPutA PRODH domain + FAD + propargylglycine <br />
+**[[2rbf]] – EcPutA DNA-binding domain + DNA<br />
+**[[4o8a]] – EcPutA DNA-binding + PRODH domains + FAD <br />
+**[[4h6q]] – DrPutA + FAD + THF <br />
+**[[3haz]] - PutA + FAD + NAD - ''Bradyrhyzobium japonicum''<br />
+**[[5ux5]] - PutA + FAD + NAD - ''Corynebacterium freiburgense''<br />
+**[[5kf6]], [[5kf7]], [[6ufp]], [[6vz9]] - PutA + FAD + NAD + THF - ''Sinorhizobium meliloti''<br />
+**[[2jxi]] - PpPutA DNA-binding domain + DNA<br />
+**[[5ur2]] - PutA + FAD derivative + inhibitor - ''Bdellovibrio bacteriovorus''<br />
+**[[6x9a]], [[6x9b]], [[6x9c]], [[6x9d]] – SmPutA + FAD + proline derivative <br />
+**[[6x99]], [[9bbo]] – SmPutA + FAD + Glu derivative <br />
+**[[9e5w]] – SmPutA + NAD + propargylglycine <br />
+**[[9c8c]] – SmPutA + NAD + FAD + allylgylglycine <br />
+**[[9c35]], [[9c36]] – SmPutA + NAD + FAD + Glu derivative<br />
+**[[9c34]] – SmPutA + NAD + FAD + Pro<br />
+**[[9dl8]], [[9dl9]], [[9e0a]], [[9e0b]], [[9e0c]], [[9e0d]], [[9e0e]] – SmPutA + NAD + FAD + alcohol<br />
+**[[9dl7]] – SmPutA + NAD + FAD + urea derivative<br />
+**[[9dl2]], [[9dl3]], [[9dl6]] – SmPutA + NAD + FAD + carboxylic acid derivative<br />
+**[[9dl4]] – SmPutA + NAD + FAD + pyridinethiol<br />
+**[[9dl5]] – SmPutA + NAD + FAD + indanone derivative<br />
+**[[7my9]], [[7mya]], [[7myb]], [[7myc]] – SmPutA + FAD + dithiolane <br />
+**[[6bsn]] – BdPutA + FAD + proline<br />
+}}
 == References ==
 <references/>
+[[Category:Topic Page]]

Proline utilization A: Difference between revisions

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