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==X-ray crystal structure of Marinitoga piezophila Argonaute in complex with 5' OH guide RNA==
==X-ray crystal structure of Marinitoga piezophila Argonaute in complex with 5' OH guide RNA==
<StructureSection load='5i4a' size='340' side='right' caption='[[5i4a]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='5i4a' size='340' side='right'caption='[[5i4a]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5i4a]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5I4A FirstGlance]. <br>
<table><tr><td colspan='2'>[[5i4a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Marinitoga_piezophila_KA3 Marinitoga piezophila KA3] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5I4A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5I4A FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5i4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4a OCA], [http://pdbe.org/5i4a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5i4a RCSB], [http://www.ebi.ac.uk/pdbsum/5i4a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4a ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.949&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5i4a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5i4a OCA], [https://pdbe.org/5i4a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5i4a RCSB], [https://www.ebi.ac.uk/pdbsum/5i4a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5i4a ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/H2J4R4_MARPK H2J4R4_MARPK]
Eukaryotic Argonaute proteins induce gene silencing by small RNA-guided recognition and cleavage of mRNA targets. Although structural similarities between human and prokaryotic Argonautes are consistent with shared mechanistic properties, sequence and structure-based alignments suggested that Argonautes encoded within CRISPR-cas[clustered regularly interspaced short palindromic repeats (CRISPR)-associated] bacterial immunity operons have divergent activities. We show here that the CRISPR-associatedMarinitoga piezophilaArgonaute (MpAgo) protein cleaves single-stranded target sequences using 5'-hydroxylated guide RNAs rather than the 5'-phosphorylated guides used by all known Argonautes. The 2.0-A resolution crystal structure of an MpAgo-RNA complex reveals a guide strand binding site comprising residues that block 5' phosphate interactions. Using structure-based sequence alignment, we were able to identify other putative MpAgo-like proteins, all of which are encoded within CRISPR-casloci. Taken together, our data suggest the evolution of an Argonaute subclass with noncanonical specificity for a 5'-hydroxylated guide.


A bacterial Argonaute with noncanonical guide RNA specificity.,Kaya E, Doxzen KW, Knoll KR, Wilson RC, Strutt SC, Kranzusch PJ, Doudna JA Proc Natl Acad Sci U S A. 2016 Mar 30. pii: 201524385. PMID:27035975<ref>PMID:27035975</ref>
==See Also==
 
*[[Argonaute 3D structures|Argonaute 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5i4a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Doudna, J A]]
[[Category: Large Structures]]
[[Category: Doxzen, K W]]
[[Category: Marinitoga piezophila KA3]]
[[Category: Kaya, E]]
[[Category: Synthetic construct]]
[[Category: Knoll, K R]]
[[Category: Doudna JA]]
[[Category: Kranzusch, P J]]
[[Category: Doxzen KW]]
[[Category: Strutt, S C]]
[[Category: Kaya E]]
[[Category: Wilson, R C]]
[[Category: Knoll KR]]
[[Category: 5' oh]]
[[Category: Kranzusch PJ]]
[[Category: Argonaute]]
[[Category: Strutt SC]]
[[Category: Guide rna]]
[[Category: Wilson RC]]
[[Category: Rna binding protein-rna complex]]
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