5e4r: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:


==Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD==
==Crystal structure of domain-duplicated synthetic class II ketol-acid reductoisomerase 2Ia_KARI-DD==
<StructureSection load='5e4r' size='340' side='right' caption='[[5e4r]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
<StructureSection load='5e4r' size='340' side='right'caption='[[5e4r]], [[Resolution|resolution]] 1.94&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5e4r]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E4R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5E4R FirstGlance]. <br>
<table><tr><td colspan='2'>[[5e4r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ignisphaera_aggregans_DSM_17230 Ignisphaera aggregans DSM 17230]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5E4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5E4R FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=40E:OXO(PROPAN-2-YLAMINO)ACETIC+ACID'>40E</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ketol-acid_reductoisomerase Ketol-acid reductoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.86 1.1.1.86] </span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=40E:OXO(PROPAN-2-YLAMINO)ACETIC+ACID'>40E</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5e4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e4r OCA], [http://pdbe.org/5e4r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5e4r RCSB], [http://www.ebi.ac.uk/pdbsum/5e4r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5e4r ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5e4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5e4r OCA], [https://pdbe.org/5e4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5e4r RCSB], [https://www.ebi.ac.uk/pdbsum/5e4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5e4r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ILVC_IGNAA ILVC_IGNAA] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH or NADH to yield (R)-2,3-dihydroxy-isovalerate.<ref>PMID:25172159</ref> <ref>PMID:26644020</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
Line 17: Line 19:
</div>
</div>
<div class="pdbe-citations 5e4r" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 5e4r" style="background-color:#fffaf0;"></div>
==See Also==
*[[Ketol-acid reductoisomerase 3D structures|Ketol-acid reductoisomerase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Ketol-acid reductoisomerase]]
[[Category: Ignisphaera aggregans DSM 17230]]
[[Category: Arnold, F H]]
[[Category: Large Structures]]
[[Category: Brinkmann-Chen, S]]
[[Category: Arnold FH]]
[[Category: Buller, A R]]
[[Category: Brinkmann-Chen S]]
[[Category: Cahn, J K.B]]
[[Category: Buller AR]]
[[Category: Chimera protein]]
[[Category: Cahn JKB]]
[[Category: Ketol-acid reductiosomerase]]
[[Category: Oxidoreductase]]
[[Category: Protein engineering]]
[[Category: Protein knot]]
[[Category: Psuedodimer]]
Retrieved from "https://proteopedia.org/5e4r"