5lfi: Difference between revisions
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New page: '''Unreleased structure''' The entry 5lfi is ON HOLD Authors: Persson, C., Fuochi, V., Pedersen, A., Karlsson, B.G., Nissen-Meyer, J., Kristiansen, P.E., Oppegard, C. Description: lact... |
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The | ==lactococcin A immunity protein== | ||
<StructureSection load='5lfi' size='340' side='right'caption='[[5lfi]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5lfi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_lactis_subsp._lactis Lactococcus lactis subsp. lactis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5LFI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5LFI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5lfi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5lfi OCA], [https://pdbe.org/5lfi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5lfi RCSB], [https://www.ebi.ac.uk/pdbsum/5lfi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5lfi ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LCIA_LACLL LCIA_LACLL] Imparts immunity to lactococcin-A to naturally sensitive host strains. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The class IId bacteriocin lactococcin A and the pediocin-like bacteriocins induce membrane leakage and cell death by specifically binding the mannose phophotransferase system (man-PTS) on their target cells. The bacteriocins' cognate immunity proteins that protect the producer cell from its own bacteriocin recognize and bind to the bacteriocin-man-PTS complex and thereby block membrane leakage. In this study, we have determined the three-dimensional structure of the lactococcin A immunity protein (LciA) by the use of nuclear magnetic resonance spectroscopy. LciA forms a four-helix bundle structure with a flexible C-terminal tail. Despite the low degree of sequence similarity between LciA and the pediocin-like immunity proteins, they share the same fold. However, there are certain differences between the structures. The C-terminal helix in LciA is considerably shorter than that observed in the pediocin-like immunity proteins, and the surface potentials of the immunity proteins differ. Truncated variants of LciA in which 6 or 10 of the C-terminal residues were removed yielded a reduced degree of protection, indicating that the unstructured C-terminal tail is important for the functionality of the immunity proteins. | |||
Nuclear Magnetic Resonance Structure and Mutational Analysis of the Lactococcin A Immunity Protein.,Kristiansen PE, Persson C, Fuochi V, Pedersen A, Karlsson GB, Nissen-Meyer J, Oppegard C Biochemistry. 2016 Nov 3. PMID:27808503<ref>PMID:27808503</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Fuochi | <div class="pdbe-citations 5lfi" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Lactococcus lactis subsp. lactis]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Fuochi V]] | |||
[[Category: Karlsson BG]] | |||
[[Category: Kristiansen PE]] | |||
[[Category: Nissen-Meyer J]] | |||
[[Category: Oppegard C]] | |||
[[Category: Pedersen A]] | |||
[[Category: Persson C]] | |||
Latest revision as of 06:02, 19 June 2024
lactococcin A immunity protein
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